ALT1/YLR089C Summary Help

Standard Name ALT1 1, 2
Systematic Name YLR089C
Feature Type ORF, Verified
Description Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthesis and catabolism; TOR1-independent role in determining chronological lifespan; expression is induced in the presence of alanine; repression is mediated by Nrg1p; ALT1 has a paralog, ALT2, that arose from the whole genome duplication; Alt2p is catalytically inactive (2, 3, 4, 5, 6)
Name Description ALanine Transaminase 2
Chromosomal Location
ChrXII:320015 to 318237 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All ALT1 GO evidence and references
  View Computational GO annotations for ALT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 148 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
212 total interaction(s) for 154 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 2
  • PCA: 1
  • Two-hybrid: 2

Genetic Interactions
  • Negative Genetic: 135
  • Positive Genetic: 53
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 592
Molecular Weight (Da) 66,421
Isoelectric Point (pI) 7.01
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:320015 to 318237 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1779 320015..318237 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004079
References cited on this page View Complete Literature Guide for ALT1
1) Anaya V, et al.  (2003) personal communication
2) Blank LM, et al.  (2005) Metabolic-flux and network analysis in fourteen hemiascomycetous yeasts. FEMS Yeast Res 5(6-7):545-58
3) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4) Garcia-Campusano F, et al.  (2009) ALT1-encoded alanine aminotransferase plays a central role in the metabolism of alanine in Saccharomyces cerevisiae. Can J Microbiol 55(4):368-74
5) Penalosa-Ruiz G, et al.  (2012) Paralogous ALT1 and ALT2 Retention and Diversification Have Generated Catalytically Active and Inactive Aminotransferases in Saccharomyces cerevisiae. PLoS One 7(9):e45702
6) Yu SL, et al.  (2013) Alanine-metabolizing enzyme alt1 is critical in determining yeast life span, as revealed by combined metabolomic and genetic studies. J Proteome Res 12(4):1619-27