BOS1/YLR078C Summary Help

BOS1 BASIC INFORMATION

Standard Name BOS1 1, 2
Systematic Name YLR078C
Alias SEC32
Feature Type ORF, Verified
Description v-SNARE (vesicle specific SNAP receptor), localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi (3 and see Summary Paragraph)
Name Description Bet One Suppressor 2
GO Annotations All BOS1 GO evidence and references
    View Computational GO annotations for BOS1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All BOS1 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions BOS1 All interactions details and references
74 total interaction(s) for 31 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 23
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 23
  • Two-hybrid: 6
Genetic Interactions
  • Dosage Rescue: 11
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 2
Sequence Information
ChrXII:286560 to 285737 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 1996-07-31 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..3 286560..286558 1996-07-31 1996-07-31
Intron 4..92 286557..286469 1996-07-31 1996-07-31
CDS 93..824 286468..285737 1996-07-31 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000004068

BOS1 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for BOS1

SUMMARY PARAGRAPH for BOS1

BOS1 encodes an essential v-SNARE (a.k.a. v-SNAP receptor, a soluble NSF[N-ethylmaleimide-sensitive fusion protein] attachment protein receptor) involved in forward transport between the endoplasmic reticulum (ER) and the Golgi (4, 3, 5). Bos1p is an integral ER membrane protein, and can be found on 50nm vesicles budded from the ER (6). Regions of the v-SNAREs Bos1p and Sec22p that are homologous to mammalian synaptobrevins bind to the t-SNARE Sed5p, which has similarity to mammalian syntaxin (7). Bos1p and Sec22p interact in a Ypt1p(Rab-like GTPase)-dependent manner (4). BOS1 was identified as a suppressor of mutations in the v-SNARE Bet1p, and both Bos1p and Bet1p interact with COPII, the coat protein complex found on ER transport vesicles (2, 8). The interaction between Bos1p and Bet1p is also dependent on Ypt1p (9). Antibodies against Bos1p block protein export after vesicles bud from the ER but prior to fusion with the Golgi (6).

Last updated: 2007-07-13

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for BOS1]

1) Shim J, et al.  (1991) The BOS1 gene encodes an essential 27-kD putative membrane protein that is required for vesicular transport from the ER to the Golgi complex in yeast. J Cell Biol 113(1):55-64
2) Newman AP, et al.  (1990) BET1, BOS1, and SEC22 are members of a group of interacting yeast genes required for transport from the endoplasmic reticulum to the Golgi complex. Mol Cell Biol 10(7):3405-14
3) Ossipov D, et al.  (1999) Yeast ER-Golgi v-SNAREs Bos1p and Bet1p differ in steady-state localization and targeting. J Cell Sci 112 ( Pt 22):4135-42
4) Lian JP, et al.  (1994) Ypt1p implicated in v-SNARE activation. Nature 372(6507):698-701
5) Spang A and Schekman R  (1998) Reconstitution of retrograde transport from the Golgi to the ER in vitro. J Cell Biol 143(3):589-99
6) Lian JP and Ferro-Novick S  (1993) Bos1p, an integral membrane protein of the endoplasmic reticulum to Golgi transport vesicles, is required for their fusion competence. Cell 73(4):735-45
7) Sacher M, et al.  (1997) The synaptobrevin-related domains of Bos1p and Sec22p bind to the syntaxin-like region of Sed5p. J Biol Chem 272(27):17134-8
8) Springer S and Schekman R  (1998) Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs. Science 281(5377):698-700
9) Stone S, et al.  (1997) Bet1p activates the v-SNARE Bos1p. Mol Biol Cell 8(7):1175-81