Standard Name	BUD20 1
Systematic Name	YLR074C
Feature Type	ORF, Verified
Description	C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593 (1, 2)
Name Description	BUD site selection 1

Chromosomal Location	ChrXII:282456 to 281956 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for BUD20
Molecular Function
Manually curated	ribosomal large subunit binding (IDA)
Biological Process
Manually curated	ribosomal large subunit export from nucleus (IGI, IMP)
High-throughput	cellular bud site selection (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) nucleus (IDA) preribosome, large subunit precursor (IDA)

Classical genetics
null	Rpl25p-GFP distribution: abnormal vegetative growth: decreased
Large-scale survey
null	auxotrophy budding pattern: abnormal cell cycle progression in G1 phase: delayed cell shape: abnormal cell size: decreased glycogen accumulation: increased chitin deposition: normal competitive fitness: decreased fermentative growth: decreased rate oxidative stress resistance: decreased resistance to sirolimus: increased resistance to wortmannin: increased resistance to cycloheximide: decreased resistance to tirapazamine: decreased resistance to doxorubicin: decreased stress resistance: increased vegetative growth: decreased rate viable
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	149 total interaction(s) for 86 unique genes/features.
Physical Interactions	Affinity Capture-MS: 125 Affinity Capture-RNA: 2 Affinity Capture-Western: 6 Co-localization: 1 Co-purification: 1
Genetic Interactions	Phenotypic Enhancement: 1 Synthetic Growth Defect: 4 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 8
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:282456 to 281956 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..501 282456..281956 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB382 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain M22 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000004064