RGR1/YLR071C Summary Help

Standard Name RGR1 1
Systematic Name YLR071C
Alias MED14 2
Feature Type ORF, Verified
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation (1, 3, 4)
Name Description Resistant to Glucose Repression 5
Chromosomal Location
ChrXII:278626 to 275378 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: 70 cM
Gene Ontology Annotations All RGR1 GO evidence and references
  View Computational GO annotations for RGR1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 408 genes
Regulators 1 genes
Resources
Classical genetics
null
overexpression
unspecified
Large-scale survey
conditional
null
overexpression
reduction of function
Resources
215 total interaction(s) for 67 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 108
  • Affinity Capture-Western: 63
  • Biochemical Activity: 1
  • Co-purification: 18
  • Reconstituted Complex: 6
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Phenotypic Enhancement: 4
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 6
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,082
Molecular Weight (Da) 123,357
Isoelectric Point (pI) 9.3
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:278626 to 275378 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: 70 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3249 278626..275378 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004061
References cited on this page View Complete Literature Guide for RGR1
1) Sakai A, et al.  (1990) Structure and molecular analysis of RGR1, a gene required for glucose repression of Saccharomyces cerevisiae. Mol Cell Biol 10(8):4130-8
2) Bourbon HM, et al.  (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3) Myers LC, et al.  (1998) The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev 12(1):45-54
4) Li Y, et al.  (1995) Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme. Proc Natl Acad Sci U S A 92(24):10864-8
5) Sakai A, et al.  (1988) Isolation and characterization of mutants which show an oversecretion phenotype in Saccharomyces cerevisiae. Genetics 119(3):499-506