FRE8/YLR047C Summary

Standard Name	FRE8 1
Systematic Name	YLR047C
Feature Type	ORF, Verified
Description	Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p (1, 2 and see Summary Paragraph)

Chromosomal Location	ChrXII:241407 to 239347 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All FRE8 GO evidence and references

	View Computational GO annotations for FRE8
Molecular Function
Manually curated	oxidoreductase activity, oxidizing metal ions (IMP, ISS)
Biological Process
Manually curated	cellular metal ion homeostasis (IMP)
Cellular Component
Manually curated	membrane (ISS)
High-throughput	integral to membrane (ISM)

Mutant phenotypes All FRE8 Phenotype evidence and references

Large-scale survey
null	acid pH resistance: decreased bud morphology: abnormal competitive fitness: increased resistance to 1,4-dithiothreitol: decreased resistance to L-canavanine: increased resistance to tunicamycin: decreased resistance to cadmium dichloride: decreased resistance to citric acid: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All FRE8 Interaction evidence and references

	9 total interaction(s) for 9 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Biochemical Activity: 1
Genetic Interactions	Dosage Lethality: 1 Negative Genetic: 5 Positive Genetic: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All FRE8 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:241407 to 239347 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2061	241407..239347	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004037

SUMMARY PARAGRAPH for FRE8

FRE8 is part of a family of nine homologous genes involved or predicted to be involved in iron uptake that can be roughly grouped into three classes based on sequence similarity and transcriptional regulation: FRE1 and FRE7; FRE2 through FRE6; and FRE8 and YGL160W (2, 3). FRE8 and YGL160W transcription is not affected by either iron or copper (2). Mutants lacking FRE8 are unable to grow in low iron and are respiration deficient (1).

Fre1p and Fre2p are the major cell-surface iron reductases and together account for 90-98% of cell-surface reductase activity (4, 5, 6). Fre1p and Fre2p are homologous to the human gp91^phox protein (OMIM), the large subunit of human cytochrome b₅₅₈ (6, 7, 8), which reduces oxygen to bactericidal superoxide (O₂^-) on the surface of phagocytic leukocytes. Deficiency of gp91^phox causes X-linked chronic granulomatous disease (OMIM).

Last updated: 2005-08-17

References cited on this page View Complete Literature Guide for FRE8

1)	De Freitas JM, et al. (2004) Exploratory and confirmatory gene expression profiling of mac1Delta. J Biol Chem 279(6):4450-8
2)	Georgatsou E and Alexandraki D (1999) Regulated expression of the Saccharomyces cerevisiae Fre1p/Fre2p Fe/Cu reductase related genes. Yeast 15(7):573-84
3)	Martins LJ, et al. (1998) Metalloregulation of FRE1 and FRE2 homologs in Saccharomyces cerevisiae. J Biol Chem 273(37):23716-21
4)	Dancis A, et al. (1990) Genetic evidence that ferric reductase is required for iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 10(5):2294-301
5)	Anderson GJ, et al. (1992) Ferric iron reduction and iron assimilation in Saccharomyces cerevisiae. J Inorg Biochem 47(3-4):249-55
6)	Georgatsou E and Alexandraki D (1994) Two distinctly regulated genes are required for ferric reduction, the first step of iron uptake in Saccharomyces cerevisiae. Mol Cell Biol 14(5):3065-73
7)	Dancis A, et al. (1992) Ferric reductase of Saccharomyces cerevisiae: molecular characterization, role in iron uptake, and transcriptional control by iron. Proc Natl Acad Sci U S A 89(9):3869-73
8)	Shatwell KP, et al. (1996) The FRE1 ferric reductase of Saccharomyces cerevisiae is a cytochrome b similar to that of NADPH oxidase. J Biol Chem 271(24):14240-4