COX12/YLR038C Summary Help

Standard Name COX12
Systematic Name YLR038C
Feature Type ORF, Verified
Description Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly of cytochrome c oxidase but not required for activity after assembly; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV (1, 2, 3 and see Summary Paragraph)
Name Description Cytochrome c OXidase
Chromosomal Location
ChrXII:225172 to 224921 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All COX12 GO evidence and references
  View Computational GO annotations for COX12
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 2 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
Resources
156 total interaction(s) for 108 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3

Genetic Interactions
  • Negative Genetic: 141
  • Positive Genetic: 8
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 83
Molecular Weight (Da) 9,788
Isoelectric Point (pI) 5.08
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:225172 to 224921 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..252 225172..224921 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004028
SUMMARY PARAGRAPH for COX12

Cytochrome c oxidase catalyzes the terminal step in the electron transport chain involved in cellular respiration. This multisubunit enzyme of the mitochondrial inner membrane, also known as Complex IV, is composed of three core subunits encoded by the mitochondrial genome (Cox1p, Cox2p, and Cox3p) and eight additional subunits encoded by nuclear genes (Cox4p, Cox5Ap or Cox5Bp, Cox6p, Cox7p, Cox8p, Cox9p, Cox12p, and Cox13p) (4, 5, 6). The phenotype of a mutation affecting any of the genes encoding cytochrome c oxidase subunits, or any of the multiple genes required for expression or assembly of the subunits (reviewed in 7), is a decrease or block in respiratory growth. The inability to respire is not lethal since S. cerevisiae can grow by fermentation, but nonrespiring cells grow more slowly than respiratory-competent cells even on glucose-containing medium, resulting in smaller colony size. Such mutations causing "petite" colonies were first described by Ephrussi and colleagues around 1950 (8).

Last updated: 2006-08-18 Contact SGD

References cited on this page View Complete Literature Guide for COX12
1) LaMarche AE, et al.  (1992) Isolation and characterization of COX12, the nuclear gene for a previously unrecognized subunit of Saccharomyces cerevisiae cytochrome c oxidase. J Biol Chem 267(31):22473-80
2) Reinders J, et al.  (2007) Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase. Mol Cell Proteomics 6(11):1896-906
3) Voegtle FN, et al.  (2012) Intermembrane space proteome of yeast mitochondria. Mol Cell Proteomics 11(12):1840-52
4) Taanman JW and Capaldi RA  (1992) Purification of yeast cytochrome c oxidase with a subunit composition resembling the mammalian enzyme. J Biol Chem 267(31):22481-5
5) Geier BM, et al.  (1995) Kinetic properties and ligand binding of the eleven-subunit cytochrome-c oxidase from Saccharomyces cerevisiae isolated with a novel large-scale purification method. Eur J Biochem 227(1-2):296-302
6) Cooper CE, et al.  (1991) Cytochrome c oxidase: structure, function, and membrane topology of the polypeptide subunits. Biochem Cell Biol 69(9):586-607
7) Herrmann JM and Funes S  (2005) Biogenesis of cytochrome oxidase-sophisticated assembly lines in the mitochondrial inner membrane. Gene 354():43-52
8) Ephrussi B and Slonimski PP  (1955) Subcellular units involved in the synthesis of respiratory enzymes in yeast. Nature 176(4495):1207-8