MLH2/YLR035C Summary Help

Standard Name MLH2
Systematic Name YLR035C
Feature Type ORF, Verified
Description Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p (1, 2, 3 and see Summary Paragraph)
Chromosomal Location
ChrXII:214456 to 212369 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All MLH2 GO evidence and references
  View Computational GO annotations for MLH2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Large-scale survey
33 total interaction(s) for 25 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 3

Genetic Interactions
  • Negative Genetic: 9
  • Phenotypic Enhancement: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Expression Summary
Length (a.a.) 695
Molecular Weight (Da) 78,247
Isoelectric Point (pI) 8.16
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:214456 to 212369 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2088 214456..212369 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004025

MLH2, one of four MutL homologs in S. cerevisiae, is involved in mismatch repair during mitosis and meiosis and plays a role in correcting mismatches that arise during the formation of heteroduplex DNA between two homologous chromosomes during meiotic recombination (reviewed in 4, 5). Like Pms1p and Mlh3p, two other MutL homologs, Mlh2p forms a heterodimer with Mlh1p, the fourth MutL homolog (6, 7, 8, 2). Although mlh2 single mutants are resistant to some anticancer compounds (9) and are involved in the repair of specific types of mismatches (1), its role in general mismatch repair appears to be limited. In mlh2 single mutants, there is an increase in events where the 4:4 Mendelian segregation pattern of the 8 single-strands of DNA is altered to a 5:3 non-Mendelian segregation pattern, suggesting mlh2 mutants fail to repair these mismatches during meiosis (2, 3). Although mlh2 single mutants do not display a decrease in the level of cross-overs like an mlh3 single mutant, an mlh2 mlh3 double mutant displays an increase in the level of cross-overs compared to the mlh3 single mutant, suggesting that Mlh2p may play a role in the formation of heteroduplexes that lead to cross-overs (3).

Last updated: 2007-05-29 Contact SGD

References cited on this page View Complete Literature Guide for MLH2
1) Harfe BD, et al.  (2000) Discrete in vivo roles for the MutL homologs Mlh2p and Mlh3p in the removal of frameshift intermediates in budding yeast. Curr Biol 10(3):145-8
2) Wang TF, et al.  (1999) Functional specificity of MutL homologs in yeast: evidence for three Mlh1-based heterocomplexes with distinct roles during meiosis in recombination and mismatch correction. Proc Natl Acad Sci U S A 96(24):13914-9
3) Abdullah MF, et al.  (2004) A role for the MutL homologue MLH2 in controlling heteroduplex formation and in regulating between two different crossover pathways in budding yeast. Cytogenet Genome Res 107(3-4):180-90
4) Kolodner RD and Marsischky GT  (1999) Eukaryotic DNA mismatch repair. Curr Opin Genet Dev 9(1):89-96
5) Borts RH, et al.  (2000) The many faces of mismatch repair in meiosis. Mutat Res 451(1-2):129-50
6) Prolla TA, et al.  (1994) MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast. Science 265(5175):1091-3
7) Pang Q, et al.  (1997) Functional domains of the Saccharomyces cerevisiae Mlh1p and Pms1p DNA mismatch repair proteins and their relevance to human hereditary nonpolyposis colorectal cancer-associated mutations. Mol Cell Biol 17(8):4465-73
8) Flores-Rozas H and Kolodner RD  (1998) The Saccharomyces cerevisiae MLH3 gene functions in MSH3-dependent suppression of frameshift mutations. Proc Natl Acad Sci U S A 95(21):12404-9
9) Durant ST, et al.  (1999) Dependence on RAD52 and RAD1 for anticancer drug resistance mediated by inactivation of mismatch repair genes. Curr Biol 9(1):51-4