RAD5/YLR032W Summary Help

Standard Name RAD5
Systematic Name YLR032W
Alias REV2 1 , SNM2 2
Feature Type ORF, Verified
Description DNA helicase/Ubiquitin ligase; involved in error-free branch of DNA damage tolerance (DDT) pathway; proposed to promote replication fork regression during postreplication repair by template switching; stimulates synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells (3, 4, 5, 6, 7, 8, 9)
Name Description RADiation sensitive
Chromosomal Location
ChrXII:204991 to 208500 | ORF Map | GBrowse
Genetic position: 22 cM
Gene Ontology Annotations All RAD5 GO evidence and references
  View Computational GO annotations for RAD5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
reduction of function
Large-scale survey
365 total interaction(s) for 159 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 6
  • Biochemical Activity: 3
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 23

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 93
  • Phenotypic Enhancement: 32
  • Phenotypic Suppression: 11
  • Positive Genetic: 11
  • Synthetic Growth Defect: 141
  • Synthetic Lethality: 8
  • Synthetic Rescue: 22

Expression Summary
Length (a.a.) 1,169
Molecular Weight (Da) 134,001
Isoelectric Point (pI) 6.29
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:204991 to 208500 | ORF Map | GBrowse
Genetic position: 22 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3510 204991..208500 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004022
References cited on this page View Complete Literature Guide for RAD5
1) Lemontt JF  (1971) Mutants of yeast defective in mutation induced by ultraviolet light. Genetics 68(1):21-33
2) Siede W and Brendel M  (1981) Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard. Curr Genet 4(2):145-9
3) Torres-Ramos CA, et al.  (2002) Requirement of RAD5 and MMS2 for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol 22(7):2419-26
4) Kiakos K, et al.  (2002) Saccharomyces cerevisiae RAD5 influences the excision repair of DNA minor groove adducts. J Biol Chem 277(46):44576-81
5) Blastyak A, et al.  (2007) Yeast rad5 protein required for postreplication repair has a DNA helicase activity specific for replication fork regression. Mol Cell 28(1):167-75
6) Carlile CC, et al.  (2009) Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5. J Biol Chem 284(43):29326-34
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Kuang L, et al.  (2013) A non-catalytic function of Rev1 in translesion DNA synthesis and mutagenesis is mediated by its stable interaction with Rad5. DNA Repair (Amst) 12(1):27-37
9) Fallet E, et al.  (2014) Length-dependent processing of telomeres in the absence of telomerase. Nucleic Acids Res 42(6):3648-65