RAD5/YLR032W Summary

RAD5 BASIC INFORMATION

Standard Name	RAD5
Systematic Name	YLR032W
Alias	REV2 1 , SNM2
Feature Type	ORF, Verified
Description	DNA helicase proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p (2, 3, 4, 5)
Name Description	RADiation sensitive

GO Annotations	All RAD5 GO evidence and references
	View Computational GO annotations for RAD5
Molecular Function
Manually curated	DNA-dependent ATPase activity (IDA) four-way junction DNA binding (IDA) four-way junction helicase activity (IDA) Y-form DNA binding (IDA)
Biological Process
Manually curated	double-strand break repair (IGI, IMP) free ubiquitin chain polymerization (IDA) postreplication repair (IDA) protein polyubiquitination (IDA)
Cellular Component
Manually curated	nuclear chromatin (IDA)

Mutant Phenotype	All RAD5 Phenotype details and references
Classical genetics
null	UV resistance: decreased chromosome/plasmid maintenance: abnormal resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyrimidine: decreased resistance to selenomethionine: decreased resistance to selenite(2-): decreased
reduction of function	mutation frequency: decreased resistance to 4-nitroquinoline N-oxide: decreased resistance to 4-nitroquinoline N-oxide: increased
unspecified	UV resistance: decreased X ray resistance: decreased
Large-scale survey
null	UV resistance: decreased competitive fitness: decreased resistance to hydroxyurea: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyrimidine: decreased resistance to methyl methanesulfonate: decreased resistance to cisplatin: decreased telomere length: increased viable

Interactions	RAD5 All interactions details and references
	215 total interaction(s) for 110 unique genes/features.
Physical Interactions	Affinity Capture-MS: 3 Affinity Capture-RNA: 1 Affinity Capture-Western: 4 Biochemical Activity: 2 Reconstituted Complex: 1 Two-hybrid: 19
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Phenotypic Enhancement: 57 Phenotypic Suppression: 8 Synthetic Growth Defect: 97 Synthetic Lethality: 12 Synthetic Rescue: 9

Sequence Information

ChrXII:204992 to 208501 | |

Genetic position: 22 cM

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3510	204992..208501	1996-07-31	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000004022

RAD5 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for RAD5

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RAD5]

1)	Lemontt JF (1971) Mutants of Yeast Defective in Mutation Induced by Ultraviolet Light. Genetics 68(1):21-33
2)	Torres-Ramos CA, et al. (2002) Requirement of RAD5 and MMS2 for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol 22(7):2419-26
3)	Kiakos K, et al. (2002) Saccharomyces cerevisiae RAD5 influences the excision repair of DNA minor groove adducts. J Biol Chem 277(46):44576-81
4)	Blastyak A, et al. (2007) Yeast rad5 protein required for postreplication repair has a DNA helicase activity specific for replication fork regression. Mol Cell 28(1):167-75
5)	Carlile CM, et al. (2009) Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5. J Biol Chem 284(43):29326-34

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