RAD5/YLR032W Summary Help

Standard Name RAD5
Systematic Name YLR032W
Alias REV2 1 , SNM2 2
Feature Type ORF, Verified
Description DNA helicase/Ubiquitin ligase; involved in error-free branch of DNA damage tolerance (DDT) pathway; proposed to promote replication fork regression during postreplication repair by template switching; stimulates synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress; associates with native telomeres, cooperates with homologous recombination in senescent cells (3, 4, 5, 6, 7, 8, 9)
Name Description RADiation sensitive
Chromosomal Location
ChrXII:204991 to 208500 | ORF Map | GBrowse
Genetic position: 22 cM
Gene Ontology Annotations All RAD5 GO evidence and references
  View Computational GO annotations for RAD5
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 101 genes
Classical genetics
reduction of function
Large-scale survey
356 total interaction(s) for 156 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 6
  • Biochemical Activity: 3
  • Co-purification: 1
  • Reconstituted Complex: 3
  • Two-hybrid: 21

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 93
  • Phenotypic Enhancement: 32
  • Phenotypic Suppression: 9
  • Positive Genetic: 11
  • Synthetic Growth Defect: 138
  • Synthetic Lethality: 8
  • Synthetic Rescue: 21

Expression Summary
Length (a.a.) 1,169
Molecular Weight (Da) 134,001
Isoelectric Point (pI) 6.29
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:204991 to 208500 | ORF Map | GBrowse
Genetic position: 22 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3510 204991..208500 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004022
References cited on this page View Complete Literature Guide for RAD5
1) Lemontt JF  (1971) Mutants of yeast defective in mutation induced by ultraviolet light. Genetics 68(1):21-33
2) Siede W and Brendel M  (1981) Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard. Curr Genet 4(2):145-9
3) Torres-Ramos CA, et al.  (2002) Requirement of RAD5 and MMS2 for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol 22(7):2419-26
4) Kiakos K, et al.  (2002) Saccharomyces cerevisiae RAD5 influences the excision repair of DNA minor groove adducts. J Biol Chem 277(46):44576-81
5) Blastyak A, et al.  (2007) Yeast rad5 protein required for postreplication repair has a DNA helicase activity specific for replication fork regression. Mol Cell 28(1):167-75
6) Carlile CM, et al.  (2009) Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5. J Biol Chem 284(43):29326-34
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
8) Kuang L, et al.  (2013) A non-catalytic function of Rev1 in translesion DNA synthesis and mutagenesis is mediated by its stable interaction with Rad5. DNA Repair (Amst) 12(1):27-37
9) Fallet E, et al.  (2014) Length-dependent processing of telomeres in the absence of telomerase. Nucleic Acids Res 42(6):3648-65