RAD5/YLR032W Summary

RAD5/YLR032W Summary

Standard Name	RAD5
Systematic Name	YLR032W
Alias	REV2 1 , SNM2
Feature Type	ORF, Verified
Description	DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress (2, 3, 4, 5, 6, 7)
Name Description	RADiation sensitive

Chromosomal Location	ChrXII:204991 to 208500 \| ORF Map \| GBrowse

	Genetic position: 22 cM

Gene Ontology Annotations All RAD5 GO evidence and references

	View Computational GO annotations for RAD5
Molecular Function
Manually curated	DNA-dependent ATPase activity (IDA) four-way junction DNA binding (IDA) four-way junction helicase activity (IDA) Y-form DNA binding (IDA)
Biological Process
Manually curated	double-strand break repair (IGI, IMP) error-prone translesion synthesis (IMP) free ubiquitin chain polymerization (IDA) postreplication repair (IDA) protein polyubiquitination (IDA)
Cellular Component
Manually curated	nuclear chromatin (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant phenotypes All RAD5 Phenotype evidence and references

Classical genetics
null	UV resistance: decreased chromosome/plasmid maintenance: abnormal mutation frequency: increased resistance to methyl methanesulfonate: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyrimidine: decreased resistance to selenomethionine: decreased resistance to selenite(2-): decreased resistance to selenium(2+): decreased resistance to hydroxyurea: decreased resistance to 4-aminofolic acid: decreased
reduction of function	mutation frequency: decreased resistance to 4-nitroquinoline N-oxide: decreased resistance to 4-nitroquinoline N-oxide: increased
unspecified	UV resistance: decreased X ray resistance: decreased
Large-scale survey
null	UV resistance: decreased cell size: increased competitive fitness: decreased gamma ray resistance: decreased haploproficient heat sensitivity: increased resistance to methyl methanesulfonate: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased resistance to sodium selenide: decreased resistance to hydroxyurea: decreased resistance to fenpropimorph: decreased resistance to cycloheximide: decreased resistance to bleomycin: decreased resistance to formaldehyde: decreased resistance to tirapazamine: decreased resistance to mycophenolic acid: increased resistance to fluconazole: increased resistance to cisplatin: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyrimidine: decreased resistance to glycolaldehyde: decreased starvation resistance: decreased telomere length: increased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All RAD5 Interaction evidence and references

	352 total interaction(s) for 156 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Biochemical Activity: 3 Co-purification: 1 Reconstituted Complex: 3 Two-hybrid: 21
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 93 Phenotypic Enhancement: 32 Phenotypic Suppression: 9 Positive Genetic: 11 Synthetic Growth Defect: 136 Synthetic Lethality: 8 Synthetic Rescue: 21
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All RAD5 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:204991 to 208500 | |

SGD ORF map

: 22 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3510	204991..208500	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000004022

References cited on this page View Complete Literature Guide for RAD5

1)	Lemontt JF (1971) Mutants of yeast defective in mutation induced by ultraviolet light. Genetics 68(1):21-33
2)	Torres-Ramos CA, et al. (2002) Requirement of RAD5 and MMS2 for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol 22(7):2419-26
3)	Kiakos K, et al. (2002) Saccharomyces cerevisiae RAD5 influences the excision repair of DNA minor groove adducts. J Biol Chem 277(46):44576-81
4)	Blastyak A, et al. (2007) Yeast rad5 protein required for postreplication repair has a DNA helicase activity specific for replication fork regression. Mol Cell 28(1):167-75
5)	Carlile CM, et al. (2009) Synthesis of free and proliferating cell nuclear antigen-bound polyubiquitin chains by the RING E3 ubiquitin ligase Rad5. J Biol Chem 284(43):29326-34
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Kuang L, et al. (2013) A non-catalytic function of Rev1 in translesion DNA synthesis and mutagenesis is mediated by its stable interaction with Rad5. DNA Repair (Amst) 12(1):27-37