SDO1/YLR022C Summary Help

Standard Name SDO1 1
Systematic Name YLR022C
Feature Type ORF, Verified
Description Guanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Chromosomal Location
ChrXII:184926 to 184174 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All SDO1 GO evidence and references
  View Computational GO annotations for SDO1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
reduction of function
127 total interaction(s) for 119 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 12
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • PCA: 1
  • Protein-peptide: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 8

Genetic Interactions
  • Negative Genetic: 12
  • Synthetic Growth Defect: 77
  • Synthetic Lethality: 4
  • Synthetic Rescue: 3

Expression Summary
Length (a.a.) 250
Molecular Weight (Da) 28,283
Isoelectric Point (pI) 9.95
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrXII:184926 to 184174 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..753 184926..184174 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004012

SDO1 encodes a 60S ribosome maturation factor associated with pre-60S ribosomes and polysomes where it functions in the release and recycling of pre-60S constituent Tif6p. Tif6p is required for efficient processing of rRNA and for pre-60S ribosome nuclear export. Tif6p shuttles between the nucleus and cytoplasm and its release from pre-60S ribosomes is a key-step in 60S maturation and subsequent translational activation of ribosomes (1 and references therein).

Sdo1p is highly conserved among archae and eukaryotes (4). Mutations in the human ortholog, SBDS, cause Shwachman-Diamond syndrome (aka Shwachman-Bodian-Diamond syndrome), an autosomal recessive disease characterized by pancreatic insufficiency, skeletal abnormalities, bone marrow failure and a predisposition to leukemia. Another S. cerevisiae protein, YHR087W, contains the same unusual structural domain found in SBDS and orthologs, but does not display similarity beyond this domain (2 and 3).

The sdo1 deletion mutant has been variously reported both as inviable (7) and as having a slow-growth phenotype (1).

Last updated: 2007-03-26 Contact SGD

References cited on this page View Complete Literature Guide for SDO1
1) Menne TF, et al.  (2007) The Shwachman-Bodian-Diamond syndrome protein mediates translational activation of ribosomes in yeast. Nat Genet 39(4):486-95
2) Shammas C, et al.  (2005) Structural and mutational analysis of the SBDS protein family. Insight into the leukemia-associated Shwachman-Diamond Syndrome. J Biol Chem 280(19):19221-9
3) Savchenko A, et al.  (2005) The Shwachman-Bodian-Diamond syndrome protein family is involved in RNA metabolism. J Biol Chem 280(19):19213-20
4) Boocock GR, et al.  (2006) Phylogeny, sequence conservation, and functional complementation of the SBDS protein family. Genomics 87(6):758-71
5) Finch AJ, et al.  (2011) Uncoupling of GTP hydrolysis from eIF6 release on the ribosome causes Shwachman-Diamond syndrome. Genes Dev 25(9):917-29
6) Gijsbers A, et al.  (2013) Guanine nucleotide exchange in the ribosomal GTPase EFL1 is modulated by the protein mutated in the Shwachman-Diamond Syndrome. Biochem Biophys Res Commun 437(3):349-54
7) Winzeler EA, et al.  (1999) Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 285(5429):901-6