IRC25/YLR021W Summary Help

Standard Name IRC25 1, 2
Systematic Name YLR021W
Alias POC3 1 , PBA3 3 , DMP2 4
Feature Type ORF, Verified
Description Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions (1, 2, 3, 4)
Name Description Increased Recombination Centers 2
Chromosomal Location
ChrXII:183623 to 184162 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All IRC25 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
75 total interaction(s) for 51 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 11
  • Co-crystal Structure: 2
  • Co-fractionation: 3
  • Co-localization: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions
  • Negative Genetic: 22
  • Positive Genetic: 7
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 5
  • Synthetic Rescue: 6

Resources
Expression Summary
histogram
Resources
Length (a.a.) 179
Molecular Weight (Da) 20,060
Isoelectric Point (pI) 4.54
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:183623 to 184162 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..540 183623..184162 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004011
References cited on this page View Complete Literature Guide for IRC25
1) Le Tallec B, et al.  (2007) 20S proteasome assembly is orchestrated by two distinct pairs of chaperones in yeast and in mammals. Mol Cell 27(4):660-74
2) Alvaro D, et al.  (2007) Genome-wide analysis of Rad52 foci reveals diverse mechanisms impacting recombination. PLoS Genet 3(12):e228
3) Kusmierczyk AR, et al.  (2008) A multimeric assembly factor controls the formation of alternative 20S proteasomes. Nat Struct Mol Biol 15(3):237-44
4) Yashiroda H, et al.  (2008) Crystal structure of a chaperone complex that contributes to the assembly of yeast 20S proteasomes. Nat Struct Mol Biol 15(3):228-36