BRE2/YLR015W Summary Help

Standard Name BRE2
Systematic Name YLR015W
Alias CPS60 1
Feature Type ORF, Verified
Description Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L (1, 2, 3, 4, 5, 6, 7, 8)
Name Description BREfeldin A sensitivity 9
Chromosomal Location
ChrXII:175227 to 176744 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BRE2 GO evidence and references
  View Computational GO annotations for BRE2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant phenotypes All BRE2 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
Resources
interactions All BRE2 Interaction evidence and references
436 total interaction(s) for 222 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 73
  • Affinity Capture-Western: 10
  • Biochemical Activity: 5
  • Co-crystal Structure: 1
  • Co-purification: 3
  • Reconstituted Complex: 1
  • Two-hybrid: 6
Genetic Interactions
  • Dosage Growth Defect: 1
  • Negative Genetic: 222
  • Phenotypic Enhancement: 2
  • Positive Genetic: 26
  • Synthetic Growth Defect: 58
  • Synthetic Lethality: 24
  • Synthetic Rescue: 4
Resources
Expression Summary
histogram
Resources
Protein Information All BRE2 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:175227 to 176744 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1518 175227..176744 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000004005
References cited on this page View Complete Literature Guide for BRE2
1) Miller T, et al.  (2001) COMPASS: a complex of proteins associated with a trithorax-related SET domain protein. Proc Natl Acad Sci U S A 98(23):12902-7
2) Roguev A, et al.  (2001) The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J 20(24):7137-48
3) Nagy PL, et al.  (2002) A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc Natl Acad Sci U S A 99(1):90-4
4) Krogan NJ, et al.  (2002) COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. J Biol Chem 277(13):10753-5
5) Dover J, et al.  (2002) Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6. J Biol Chem 277(32):28368-71
6) Cheng H, et al.  (2004) The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3. Mol Cell Biol 24(7):2932-43
7) Askree SH, et al.  (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
8) South PF, et al.  (2010) A conserved interaction between the SDI domain of Bre2 and the Dpy-30 domain of Sdc1 is required for histone methylation and gene expression. J Biol Chem 285(1):595-607
9) Muren E, et al.  (2001) Identification of yeast deletion strains that are hypersensitive to brefeldin A or monensin, two drugs that affect intracellular transport. Yeast 18(2):163-72