COF1/YLL050C Summary Help

Standard Name COF1 1, 2
Systematic Name YLL050C
Feature Type ORF, Verified
Description Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes (1, 2, 3, 4, 5, 6, 7)
Name Description COFilin 1, 2
Gene Product Alias cofilin 1 , 2
Chromosomal Location
ChrXII:40414 to 39804 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All COF1 GO evidence and references
  View Computational GO annotations for COF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 6 genes
Resources
Classical genetics
conditional
null
overexpression
repressible
Large-scale survey
conditional
null
overexpression
reduction of function
repressible
Resources
127 total interaction(s) for 83 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 3
  • Biochemical Activity: 1
  • Co-fractionation: 4
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 7
  • Reconstituted Complex: 6
  • Two-hybrid: 9

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 26
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 2
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 13
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 143
Molecular Weight (Da) 15,901
Isoelectric Point (pI) 4.89
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrXII:40414 to 39804 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..14 40414..40401 2011-02-03 1996-07-31
Intron 15..193 40400..40222 2011-02-03 1996-07-31
CDS 194..611 40221..39804 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003973
References cited on this page View Complete Literature Guide for COF1
1) Moon AL, et al.  (1993) Cofilin is an essential component of the yeast cortical cytoskeleton. J Cell Biol 120(2):421-35
2) Iida K, et al.  (1993) Isolation of a yeast essential gene, COF1, that encodes a homologue of mammalian cofilin, a low-M(r) actin-binding and depolymerizing protein. Gene 124(1):115-20
3) Ayscough KR, et al.  (1997) High rates of actin filament turnover in budding yeast and roles for actin in establishment and maintenance of cell polarity revealed using the actin inhibitor latrunculin-A. J Cell Biol 137(2):399-416
4) Moriyama K, et al.  (1996) Phosphorylation of Ser-3 of cofilin regulates its essential function on actin. Genes Cells 1(1):73-86
5) Theriot JA  (1997) Accelerating on a treadmill: ADF/cofilin promotes rapid actin filament turnover in the dynamic cytoskeleton. J Cell Biol 136(6):1165-8
6) Wriggers W, et al.  (1998) Cofilin and gelsolin segment-1: molecular dynamics simulation and biochemical analysis predict a similar actin binding mode. J Mol Biol 282(5):921-32
7) Curwin AJ, et al.  (2012) Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Mol Biol Cell 23(12):2327-38