VPS13/YLL040C Summary

VPS13/YLL040C Summary

Standard Name	VPS13 1, 2
Systematic Name	YLL040C
Alias	SOI1 3 , VPT2 4
Feature Type	ORF, Verified
Description	Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively (3, 5, 6)
Name Description	Vacuolar Protein Sorting 1, 2

Chromosomal Location	ChrXII:63645 to 54211 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All VPS13 GO evidence and references

	View Computational GO annotations for VPS13
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	ascospore-type prospore membrane assembly (IMP) late endosome to vacuole transport (IGI, IMP) protein retention in Golgi apparatus (IMP) protein targeting to vacuole (IMP)
Cellular Component
Manually curated	extrinsic to membrane (IDA) prospore membrane (IDA)
High-throughput	colocalizes_with endosome (IDA) mitochondrion (IDA)

Mutant phenotypes All VPS13 Phenotype evidence and references

Classical genetics
null	carboxypeptidase Y secretion: decreased Vps10p transport: decreased Ste13p transport: decreased Kex2p transport: decreased sporulation: absent viable
reduction of function	vacuolar transport: abnormal
Large-scale survey
null	glutathione excretion: increased inositol excretion: increased competitive fitness: decreased resistance to camptothecin: increased resistance to hygromycin B: decreased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to mycophenolic acid: decreased resistance to sirolimus: decreased resistance to dimethyl sulfoxide: decreased resistance to mefloquine: decreased resistance to hydroxyurea: increased respiratory growth: absent sporulation: absent sporulation efficiency: decreased viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All VPS13 Interaction evidence and references

	219 total interaction(s) for 149 unique genes/features.
Physical Interactions	Affinity Capture-MS: 32 Affinity Capture-RNA: 2 Two-hybrid: 1
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 156 Phenotypic Suppression: 1 Positive Genetic: 9 Synthetic Growth Defect: 12 Synthetic Lethality: 1 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All VPS13 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:63645 to 54211 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..9435	63645..54211	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003963

References cited on this page View Complete Literature Guide for VPS13

1)	Robinson JS, et al. (1988) Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases. Mol Cell Biol 8(11):4936-48
2)	Rothman JH, et al. (1989) Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae. EMBO J 8(7):2057-65
3)	Redding K, et al. (1996) Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins. Mol Cell Biol 16(11):6208-17
4)	Bankaitis VA, et al. (1986) Isolation of yeast mutants defective in protein targeting to the vacuole. Proc Natl Acad Sci U S A 83(23):9075-9
5)	Brickner JH and Fuller RS (1997) SOI1 encodes a novel, conserved protein that promotes TGN-endosomal cycling of Kex2p and other membrane proteins by modulating the function of two TGN localization signals. J Cell Biol 139(1):23-36
6)	Park JS and Neiman AM (2012) VPS13 regulates membrane morphogenesis during sporulation in Saccharomyces cerevisiae. J Cell Sci 125(Pt 12):3004-11