Standard Name	ISA1 1
Systematic Name	YLL027W
Feature Type	ORF, Verified
Description	Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins (2, 3, 4)
Name Description	Iron Sulfur Assembly

Chromosomal Location	ChrXII:87403 to 88155 | ORF Map | GBrowse

	View Computational GO annotations for ISA1
Molecular Function
Manually curated	iron ion binding (IDA) iron-sulfur cluster binding (ISA)
Biological Process
Manually curated	biotin biosynthetic process (IGI, IMP) iron-sulfur cluster assembly (IGI, IMP)
Cellular Component
Manually curated	mitochondrial matrix (IDA)

Classical genetics
null	auxotrophy iron molecular entity accumulation: increased chromosome/plasmid maintenance: abnormal Ptr2p-GFP distribution: abnormal respiratory growth: absent viable
Large-scale survey
null	proton accumulation: decreased competitive fitness: decreased heat sensitivity: increased hyperosmotic stress resistance: decreased nutrient utilization: decreased oxidative stress resistance: decreased resistance to farnesol: decreased resistance to cadmium dichloride: decreased resistance to hydroxyurea: increased resistance to amiodarone: decreased resistance to CTBT: decreased resistance to acetic acid: decreased resistance to 4-chlorophenol: decreased resistance to 2,4-dichlorophenol: decreased resistance to pentachlorophenol: decreased resistance to methyl methanesulfonate: decreased resistance to doxorubicin: decreased resistance to calcium dichloride: decreased resistance to ethanol: decreased respiratory growth: absent respiratory growth: decreased rate stress resistance: increased telomere length: decreased toxin resistance: increased toxin resistance: decreased utilization of carbon source: decreased vegetative growth: decreased rate viable
overexpression	vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	22 total interaction(s) for 21 unique genes/features.
Physical Interactions	Affinity Capture-MS: 7 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 Biochemical Activity: 1 Two-hybrid: 2
Genetic Interactions	Synthetic Growth Defect: 2 Synthetic Lethality: 2 Synthetic Rescue: 4
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:87403 to 88155 | ORF Map | GBrowse
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..753 87403..88155 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FL100 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain T73 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003950