SPA2/YLL021W Summary

SPA2/YLL021W Summary

Standard Name	SPA2 1
Systematic Name	YLL021W
Alias	FUS6 2 , PEA1 3
Feature Type	ORF, Verified
Description	Component of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; SPA2 has a paralog, SPH1, that arose from the whole genome duplication (4, 5, 6, 7)
Name Description	Spindle Pole Antigen 1

Chromosomal Location	ChrXII:100947 to 105347 \| ORF Map \| GBrowse

	Genetic position: -28 cM

Gene Ontology Annotations All SPA2 GO evidence and references

	View Computational GO annotations for SPA2
Molecular Function
Manually curated	cytoskeletal regulatory protein binding (TAS)
Biological Process
Manually curated	invasive filamentous growth (IGI) mating projection assembly (IGI) pseudohyphal growth (IMP) regulation of initiation of mating projection growth (IMP) regulation of termination of mating projection growth (IMP)
Cellular Component
Manually curated	cellular bud neck (IDA) cellular bud tip (IDA) incipient cellular bud site (IDA) mating projection tip (IDA) polarisome (IDA)

Mutant phenotypes All SPA2 Phenotype evidence and references

Classical genetics
null	SLT2-LacZ accumulation: decreased actin cytoskeleton morphology: abnormal budding index: increased budding pattern: abnormal cell fusion: decreased cell shape: abnormal calcium(2+) accumulation: decreased entry into G0 (stationary phase): abnormal mating efficiency: decreased mating projection morphology: abnormal Fig1p-Myc accumulation: increased Bud8p-GFP distribution: abnormal Fus2p-GFP distribution: abnormal pseudohyphal growth: absent resistance to staurosporine: decreased resistance to tunicamycin: decreased shmoo formation: abnormal viable
overexpression	calcium(2+) accumulation: decreased shmoo formation: abnormal
reduction of function	cell fusion: absent
unspecified	pseudohyphal growth: decreased
Large-scale survey
null	auxotrophy bud morphology: abnormal budding pattern: abnormal cell shape: abnormal chitin deposition: normal competitive fitness: increased filamentous growth: decreased invasive growth: absent resistance to clioquinol: decreased resistance to Calcofluor White: normal resistance to St. John's Wort extract: decreased resistance to fluconazole: decreased resistance to fenpropimorph: decreased resistance to caffeine: decreased resistance to cycloheximide: decreased resistance to tunicamycin: increased resistance to methyl methanesulfonate: increased resistance to paromomycin: decreased vacuolar morphology: abnormal viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SPA2 Interaction evidence and references

	161 total interaction(s) for 104 unique genes/features.
Physical Interactions	Affinity Capture-MS: 17 Affinity Capture-RNA: 1 Affinity Capture-Western: 5 Biochemical Activity: 1 Co-localization: 3 PCA: 5 Reconstituted Complex: 3 Two-hybrid: 23
Genetic Interactions	Dosage Lethality: 1 Dosage Rescue: 14 Negative Genetic: 34 Phenotypic Enhancement: 8 Phenotypic Suppression: 5 Positive Genetic: 1 Synthetic Growth Defect: 13 Synthetic Lethality: 23 Synthetic Rescue: 4
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SPA2 Protein evidence and references

Localization	Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrXII:100947 to 105347 | |

SGD ORF map

: -28 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..4401	100947..105347	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003944

References cited on this page View Complete Literature Guide for SPA2

1)	Snyder M (1989) The SPA2 protein of yeast localizes to sites of cell growth. J Cell Biol 108(4):1419-29
2)	Gammie AE, et al. (1998) Distinct morphological phenotypes of cell fusion mutants. Mol Biol Cell 9(6):1395-410
3)	Chenevert J, et al. (1994) Identification of genes required for normal pheromone-induced cell polarization in Saccharomyces cerevisiae. Genetics 136(4):1287-96
4)	Sheu YJ, et al. (1998) Spa2p interacts with cell polarity proteins and signaling components involved in yeast cell morphogenesis. Mol Cell Biol 18(7):4053-69
5)	van Drogen F and Peter M (2002) Spa2p functions as a scaffold-like protein to recruit the Mpk1p MAP kinase module to sites of polarized growth. Curr Biol 12(19):1698-703
6)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
7)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61