SDC25/YLL016W Summary

SDC25/YLL016W Summary

Standard Name	SDC25 1
Systematic Name	YLL016W
Feature Type	pseudogene
Description	Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Suppressor of cdc25

Chromosomal Location	ChrXII:112847 to 115993 \| ORF Map \| GBrowse

	Genetic position: -3 cM

Gene Ontology Annotations All SDC25 GO evidence and references

	View Computational GO annotations for SDC25
Molecular Function
Manually curated	Ras guanyl-nucleotide exchange factor activity (TAS)
Biological Process
Manually curated	Ras protein signal transduction (TAS)
Cellular Component
Manually curated	cellular_component unknown (ND)

Regulatory Role

Regulatory modules	predicted: cellcycle (353)

Mutant phenotypes All SDC25 Phenotype evidence and references

Large-scale survey
null	anaerobic growth: decreased cell size: decreased competitive fitness: increased resistance to amitrole: decreased resistance to cycloheximide: decreased starvation resistance: decreased viable
overexpression	vegetative growth: decreased
Resources	PROPHECY \| SCMD

interactions All SDC25 Interaction evidence and references

	10 total interaction(s) for 9 unique genes/features.
Physical Interactions	Co-purification: 1 PCA: 1 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 3 Dosage Rescue: 1 Negative Genetic: 2 Phenotypic Enhancement: 1
Resources	BioGRID

Expression Summary


Resources	Genevestigator \| YMGV

Protein Information All SDC25 Protein evidence and references

Localization	YeastGFP
Phosphorylation	PhosphoGRID
Structure	SCOP \| YeastRC
Homologs	PDB Homologs

sequence information

ChrXII:112847 to 115993 | |

SGD ORF map

: -3 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3147	112847..115993	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP vs. fungi \| Synteny Viewer

Resources

External Links	All Associated Seq \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003939

References cited on this page View Complete Literature Guide for SDC25

1)	Damak F, et al. (1991) SDC25, a CDC25-like gene which contains a RAS-activating domain and is a dispensable gene of Saccharomyces cerevisiae. Mol Cell Biol 11(1):202-12
2)	Boy-Marcotte E, et al. (1996) SDC25, a dispensable Ras guanine nucleotide exchange factor of Saccharomyces cerevisiae differs from CDC25 by its regulation. Mol Biol Cell 7(4):529-39
3)	Boy-Marcotte E, et al. (1989) The C-terminal part of a gene partially homologous to CDC 25 gene suppresses the cdc25-5 mutation in Saccharomyces cerevisiae. Gene 77(1):21-30
4)	Crechet JB, et al. (2000) Analysis of the role of the hypervariable region of yeast Ras2p and its farnesylation in the interaction with exchange factors and adenylyl cyclase. J Biol Chem 275(23):17754-61
5)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61