PUF3/YLL013C Summary

PUF3 BASIC INFORMATION

Standard Name	PUF3
Systematic Name	YLL013C
Feature Type	ORF, Verified
Description	Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins (1, 2, 3)
Name Description	PUmilio-homology domain Family

GO Annotations	All PUF3 GO evidence and references
	View Computational GO annotations for PUF3
Molecular Function
Manually curated	mRNA binding (IDA)
Biological Process
Manually curated	aerobic respiration (IMP) intracellular mRNA localization (IMP) mitochondrion localization (IMP) mitochondrion organization (IMP) nuclear-transcribed mRNA catabolic process (IDA) nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) external side of mitochondrial outer membrane (IDA)

Mutant Phenotype	All PUF3 Phenotype details and references
Classical genetics
null	mitochondrial morphology: abnormal mitochondrial transport: abnormal
overexpression	respiratory growth: absent
Large-scale survey
null	hyperosmotic stress resistance: decreased viable

Interactions	PUF3 All interactions details and references
	28 total interaction(s) for 22 unique genes/features.
Physical Interactions	Affinity Capture-MS: 10 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 Co-crystal Structure: 1 PCA: 1 Protein-RNA: 4 Two-hybrid: 1
Genetic Interactions	Phenotypic Enhancement: 2 Phenotypic Suppression: 3 Synthetic Growth Defect: 1 Synthetic Lethality: 1

Sequence Information

ChrXII:124713 to 122074 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 1996-07-31 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2640	124713..122074	1996-07-31	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003936

PUF3 RESOURCES

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SGD ORF map	GBrowse

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Expression Summary

ADDITIONAL INFORMATION for PUF3

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PUF3]

1)	Olivas W and Parker R (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
2)	Gerber AP, et al. (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79
3)	Garcia-Rodriguez LJ, et al. (2007) Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast. J Cell Biol 176(2):197-207

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