Standard Name	PUF3
Systematic Name	YLL013C
Feature Type	ORF, Verified
Description	Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins (1, 2, 3)
Name Description	PUmilio-homology domain Family

Chromosomal Location	ChrXII:124714 to 122075 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for PUF3
Molecular Function
Manually curated	mRNA binding (IDA)
Biological Process
Manually curated	aerobic respiration (IMP) intracellular mRNA localization (IMP) mitochondrion localization (IMP) mitochondrion organization (IMP) nuclear-transcribed mRNA catabolic process (IDA) nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (IMP)
Cellular Component
Manually curated	cytoplasm (IDA) external side of mitochondrial outer membrane (IDA)

Classical genetics
null	mitochondrial morphology: abnormal mitochondrial transport: abnormal respiratory growth: decreased
overexpression	respiratory growth: absent
Large-scale survey
null	competitive fitness: increased haploinsufficient hyperosmotic stress resistance: decreased resistance to cycloheximide: decreased resistance to amitrole: decreased starvation resistance: decreased stress resistance: decreased viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	83 total interaction(s) for 67 unique genes/features.
Physical Interactions	Affinity Capture-MS: 32 Affinity Capture-RNA: 3 Affinity Capture-Western: 2 Co-crystal Structure: 1 PCA: 1 Protein-RNA: 4 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 1 Negative Genetic: 24 Phenotypic Enhancement: 2 Positive Genetic: 10 Synthetic Growth Defect: 1 Synthetic Lethality: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrXII:124714 to 122075 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2640 124714..122075 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CEN.PK113-7D S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003936