IML1/YJR138W Summary Help

Standard Name IML1 1
Systematic Name YJR138W
Alias SEA1 2
Feature Type ORF, Verified
Description GTPase-activating protein (GAP), subunit of SEA and Iml1p complexes; SEA (Seh1-associated) complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; localized to either pre-autophagosomal structures (PAS) or non-PAS structures during NNS-induced autophagy; (2, 3, 4, 5)
Name Description Increased Minichromosome Loss 1
Chromosomal Location
ChrX:684567 to 689321 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All IML1 GO evidence and references
  View Computational GO annotations for IML1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 8 genes
Resources
Large-scale survey
null
Resources
51 total interaction(s) for 34 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 34
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 4
  • PCA: 1
  • Two-hybrid: 6

Genetic Interactions
  • Dosage Growth Defect: 2
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,584
Molecular Weight (Da) 181,948
Isoelectric Point (pI) 9.32
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:684567 to 689321 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2011-02-03
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4755 684567..689321 2011-02-03 2011-02-03
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003899
References cited on this page View Complete Literature Guide for IML1
1) Entian KD, et al.  (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702
2) Dokudovskaya S, et al.  (2011) A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics 10(6):M110.006478
3) Huh WK, et al.  (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
4) Wu X and Tu BP  (2011) Selective regulation of autophagy by the Iml1-Npr2-Npr3 complex in the absence of nitrogen starvation. Mol Biol Cell 22(21):4124-33
5) Panchaud N, et al.  (2013) Amino Acid Deprivation Inhibits TORC1 Through a GTPase-Activating Protein Complex for the Rag Family GTPase Gtr1. Sci Signal 6(277):ra42