NNF1/YJR112W Summary Help

Standard Name NNF1 1
Systematic Name YJR112W
Feature Type ORF, Verified
Description Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND) (1, 2)
Name Description Necessary for Nuclear Function 1
Chromosomal Location
ChrX:637030 to 637635 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NNF1 GO evidence and references
  View Computational GO annotations for NNF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 3 genes
Resources
Large-scale survey
conditional
null
reduction of function
Resources
207 total interaction(s) for 110 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 27
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 18
  • Biochemical Activity: 1
  • Co-crystal Structure: 1
  • Co-fractionation: 3
  • PCA: 8
  • Reconstituted Complex: 12
  • Two-hybrid: 119

Genetic Interactions
  • Dosage Rescue: 4
  • Negative Genetic: 3
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 201
Molecular Weight (Da) 23,639
Isoelectric Point (pI) 4.61
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:637030 to 637635 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..606 637030..637635 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003873
References cited on this page View Complete Literature Guide for NNF1
1) Shan X, et al.  (1997) NNF1 is an essential yeast gene required for proper spindle orientation, nucleolar and nuclear envelope structure and mRNA export. J Cell Sci 110 ( Pt 14)():1615-24
2) Euskirchen GM  (2002) Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a new group of proteins important for chromosome segregation in Saccharomyces cerevisiae. Eukaryot Cell 1(2):229-40