CPA2/YJR109C Summary Help

Standard Name CPA2
Systematic Name YJR109C
Feature Type ORF, Verified
Description Large subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor (1 and see Summary Paragraph)
Name Description Carbamyl Phosphate synthetase A
Chromosomal Location
ChrX:632941 to 629585 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All CPA2 GO evidence and references
  View Computational GO annotations for CPA2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 9 genes
Classical genetics
reduction of function
Large-scale survey
42 total interaction(s) for 31 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-RNA: 1
  • Co-purification: 1
  • Reconstituted Complex: 2

Genetic Interactions
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 1,118
Molecular Weight (Da) 123,914
Isoelectric Point (pI) 5.05
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:632941 to 629585 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3357 632941..629585 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003870

CPA2 encodes the large subunit of carbamoyl-phosphate synthase (glutamine-hydrolyzing) (1, 2), a cytosolic enzyme in the arginine biosynthetic pathway (3). The small subunit, encoded by CPA1, binds and cleaves glutamine, while the large subunit, Cpa2p, binds the remaining substrates and carries out all other reactions catalyzed by the heterodimer (4). Interaction between Cpa1p and Cpa2p increases the Vmax of Cpa2p, perhaps by causing a conformational change in Cpa2p (4). Yeast carbamoyl-phosphate synthase shares sequence similarity with heterodimeric and single subunit carbamoyl-phosphate synthases from E. coli and eukaryotes including rat, human, and hamster (1, 5, 6, 4, 2). In humans, carbamoyl-phosphate synthase deficiency causes hyperammonemia (7).

Last updated: 1999-10-22 Contact SGD

References cited on this page View Complete Literature Guide for CPA2
1) Lusty CJ, et al.  (1983) Yeast carbamyl phosphate synthetase. Structure of the yeast gene and homology to Escherichia coli carbamyl phosphate synthetase. J Biol Chem 258(23):14466-77
2) Bernard A, et al.  (1997) In vivo mutational analysis of highly conserved amino acid residues of the small subunit Cpa1p of the carbamylphosphate synthetase of Saccharomyces cerevisiae. Yeast 13(11):1021-8
3) Jauniaux JC, et al.  (1978) Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol 133(3):1096-1107
4) Lim AL and Powers-Lee SG  (1996) Requirement for the carboxyl-terminal domain of Saccharomyces cerevisiae carbamoyl-phosphate synthetase. J Biol Chem 271(19):11400-9
5) Nyunoya H, et al.  (1985) Characterization and derivation of the gene coding for mitochondrial carbamyl phosphate synthetase I of rat. J Biol Chem 260(16):9346-56
6) Reiser J, et al.  (1994) Molecular analysis of the Trichosporon cutaneum DSM 70698 argA gene and its use for DNA-mediated transformations. J Bacteriol 176(10):3021-32
7) Foury F  (1997) Human genetic diseases: a cross-talk between man and yeast. Gene 195(1):1-10