BUD4/YJR092W Summary Help

Standard Name BUD4 1
Systematic Name YJR092W
Feature Type ORF, Verified
Description Anillin-like protein involved in bud-site selection; required for the axial budding pattern; localizes with septins to the bud neck in mitosis and may constitute an axial landmark for the next round of budding; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; in vivo substrate of Cdc28p/Clb2p (1, 2, 3, 4)
Name Description BUD site selection
Chromosomal Location
ChrX:598735 to 603078 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All BUD4 GO evidence and references
  View Computational GO annotations for BUD4
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 5 genes
Resources
Classical genetics
null
reduction of function
unspecified
Large-scale survey
null
overexpression
unspecified
Resources
75 total interaction(s) for 53 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 10
  • Biochemical Activity: 9
  • Co-purification: 1
  • Reconstituted Complex: 5
  • Two-hybrid: 2

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 6
  • Phenotypic Enhancement: 7
  • Phenotypic Suppression: 3
  • Positive Genetic: 3
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 3

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,447
Molecular Weight (Da) 164,485
Isoelectric Point (pI) 4.58
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:598735 to 603078 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 2008-06-04
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4344 598735..603078 2011-02-03 2008-06-04
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003852
References cited on this page View Complete Literature Guide for BUD4
1) Chant J and Herskowitz I  (1991) Genetic control of bud site selection in yeast by a set of gene products that constitute a morphogenetic pathway. Cell 65(7):1203-12
2) Sanders SL and Herskowitz I  (1996) The BUD4 protein of yeast, required for axial budding, is localized to the mother/BUD neck in a cell cycle-dependent manner. J Cell Biol 134(2):413-27
3) Wloka C, et al.  (2011) Evidence that a septin diffusion barrier is dispensable for cytokinesis in budding yeast. Biol Chem 392(8-9):813-829
4) Eluere R, et al.  (2012) Cdk and the anillin homolog Bud4 define a new pathway regulating septin organization in yeast. Cell Cycle 11(1):151-8