JSN1/YJR091C Summary Help

Standard Name JSN1 1
Systematic Name YJR091C
Alias PUF1
Feature Type ORF, Verified
Description Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Just Say No 1
Chromosomal Location
ChrX:598336 to 595061 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All JSN1 GO evidence and references
  View Computational GO annotations for JSN1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 7 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
74 total interaction(s) for 63 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Co-localization: 1
  • PCA: 3
  • Protein-RNA: 2
  • Reconstituted Complex: 1
  • Two-hybrid: 24

Genetic Interactions
  • Dosage Rescue: 2
  • Negative Genetic: 15
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 1
  • Positive Genetic: 11
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,091
Molecular Weight (Da) 120,373
Isoelectric Point (pI) 6.96
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:598336 to 595061 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3276 598336..595061 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003851
References cited on this page View Complete Literature Guide for JSN1
1) Machin NA, et al.  (1995) Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant. Mol Biol Cell 6(9):1241-59
2) Olivas W and Parker R  (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
3) Gerber AP, et al.  (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79
4) Fehrenbacher KL, et al.  (2005) A role for Jsn1p in recruiting the Arp2/3 complex to mitochondria in budding yeast. Mol Biol Cell 16(11):5094-102
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61