Standard Name	JSN1
Systematic Name	YJR091C
Alias	PUF1
Feature Type	ORF, Verified
Description	Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description	Just Say No 1

Chromosomal Location	ChrX:598336 to 595061 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for JSN1
Molecular Function
Manually curated	mRNA binding (ISS)
High-throughput	mRNA binding (IDA)
Biological Process
Manually curated	cellular protein complex localization (IMP) establishment of mitochondrion localization (IMP) nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay (IGI)
Cellular Component
Manually curated	external side of mitochondrial outer membrane (IDA)

Classical genetics
null	mitochondrial morphology: abnormal
Large-scale survey
null	competitive fitness: decreased haploinsufficient resistance to benzo[a]pyrene: decreased vacuolar morphology: abnormal viable
overexpression	vegetative growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	74 total interaction(s) for 63 unique genes/features.
Physical Interactions	Affinity Capture-MS: 6 Affinity Capture-RNA: 2 Affinity Capture-Western: 2 Biochemical Activity: 1 Co-localization: 1 PCA: 3 Protein-RNA: 2 Reconstituted Complex: 1 Two-hybrid: 24
Genetic Interactions	Dosage Rescue: 2 Negative Genetic: 15 Phenotypic Enhancement: 2 Phenotypic Suppression: 1 Positive Genetic: 11 Synthetic Rescue: 1
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrX:598336 to 595061 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..3276 598336..595061 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain YJM789 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003851