OPI3/YJR073C Summary Help

Standard Name OPI3 1
Systematic Name YJR073C
Alias PEM2 2 , 3
Feature Type ORF, Verified
Description Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase (2, 4)
Name Description OverProducer of Inositol 1
Chromosomal Location
ChrX:572935 to 572315 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All OPI3 GO evidence and references
  View Computational GO annotations for OPI3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 7 genes
Resources
Pathways
Classical genetics
null
Large-scale survey
null
overexpression
Resources
1246 total interaction(s) for 572 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 6
  • Affinity Capture-RNA: 2
  • Protein-RNA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 2
  • Negative Genetic: 908
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 80
  • Positive Genetic: 179
  • Synthetic Growth Defect: 38
  • Synthetic Lethality: 15

Resources
Expression Summary
histogram
Resources
Length (a.a.) 206
Molecular Weight (Da) 23,150
Isoelectric Point (pI) 9.6
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:572935 to 572315 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..621 572935..572315 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003834
References cited on this page View Complete Literature Guide for OPI3
1) Greenberg ML, et al.  (1982) Regulatory mutations of inositol biosynthesis in yeast: isolation of inositol-excreting mutants. Genetics 100(1):19-33
2) Kodaki T and Yamashita S  (1987) Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes. J Biol Chem 262(32):15428-35
3) Kodaki T and Yamashita S  (1989) Characterization of the methyltransferases in the yeast phosphatidylethanolamine methylation pathway by selective gene disruption. Eur J Biochem 185(2):243-51
4) McGraw P and Henry SA  (1989) Mutations in the Saccharomyces cerevisiae opi3 gene: effects on phospholipid methylation, growth and cross-pathway regulation of inositol synthesis. Genetics 122(2):317-30