OPI3/YJR073C Summary

OPI3/YJR073C Summary

Standard Name	OPI3
Systematic Name	YJR073C
Alias	PEM2 1 , 2
Feature Type	ORF, Verified
Description	Phospholipid methyltransferase (methylene-fatty-acyl-phospholipid synthase), catalyzes the last two steps in phosphatidylcholine biosynthesis (1, 3)
Name Description	OverProducer of Inositol 4

Chromosomal Location	ChrX:572935 to 572315 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All OPI3 GO evidence and references

	View Computational GO annotations for OPI3
Molecular Function
Manually curated	phosphatidyl-N-dimethylethanolamine N-methyltransferase activity (IDA, IGI, IMP) phosphatidyl-N-methylethanolamine N-methyltransferase activity (IDA, IGI, IMP)
Biological Process
Manually curated	phosphatidylcholine biosynthetic process (IDA, IGI, IMP)
Cellular Component
Manually curated	integral to membrane (ISM)
High-throughput	mitochondrion (IDA)

Pathways	phosphatidic acid and phospholipid biosynthesis phospholipid biosynthesis superpathway of phospholipid biosynthesis

Mutant phenotypes All OPI3 Phenotype evidence and references

Classical genetics
null	phospholipid accumulation: abnormal dessication resistance: decreased endoplasmic reticulum morphology: abnormal nutrient utilization: decreased Arn1p distribution: abnormal resistance to staurosporine: decreased resistance to tunicamycin: decreased resistance to chlorpromazine: decreased resistance to ferrozine(2-): decreased resistance to cobalt chloride hexahydrate: decreased toxin resistance: decreased
Large-scale survey
null	anaerobic growth: decreased proton accumulation: increased glycogen accumulation: increased inositol excretion: increased chitin deposition: normal competitive fitness: decreased dessication resistance: decreased endocytosis: decreased filamentous growth: decreased heat sensitivity: increased invasive growth: absent lipid particle morphology: abnormal metal resistance: decreased Can1p-GFP distribution: abnormal resistance to streptomycin: decreased resistance to paromomycin: decreased resistance to amitrole: decreased resistance to cycloheximide: decreased resistance to Calcofluor White: decreased resistance to ethanol: decreased resistance to arsenite(3-): decreased resistance to sodium arsenite: decreased resistance to caffeine: decreased resistance to fenpropimorph: decreased resistance to fluconazole: decreased resistance to chloroquine: decreased resistance to mefloquine: decreased resistance to Echinacea extract: decreased stress resistance: decreased toxin resistance: decreased transposable element transposition: decreased utilization of nitrogen source: absent vegetative growth: abnormal viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All OPI3 Interaction evidence and references

	1099 total interaction(s) for 520 unique genes/features.
Physical Interactions	Affinity Capture-MS: 4 Affinity Capture-RNA: 2 Protein-RNA: 1 Reconstituted Complex: 1 Two-hybrid: 1
Genetic Interactions	Dosage Growth Defect: 1 Dosage Rescue: 2 Negative Genetic: 774 Phenotypic Enhancement: 10 Phenotypic Suppression: 80 Positive Genetic: 170 Synthetic Growth Defect: 38 Synthetic Lethality: 15
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder \| YeastRC Two-Hybrid

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All OPI3 Protein evidence and references

Localization	YeastRC \| Organelle DB (UMich) \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrX:572935 to 572315 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..621	572935..572315	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003834

References cited on this page View Complete Literature Guide for OPI3

1)	Kodaki T and Yamashita S (1987) Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes. J Biol Chem 262(32):15428-35
2)	Kodaki T and Yamashita S (1989) Characterization of the methyltransferases in the yeast phosphatidylethanolamine methylation pathway by selective gene disruption. Eur J Biochem 185(2):243-51
3)	McGraw P and Henry SA (1989) Mutations in the Saccharomyces cerevisiae opi3 gene: effects on phospholipid methylation, growth and cross-pathway regulation of inositol synthesis. Genetics 122(2):317-30
4)	Greenberg ML, et al. (1982) Regulatory mutations of inositol biosynthesis in yeast: isolation of inositol-excreting mutants. Genetics 100(1):19-33