NPA3/YJR072C Summary Help

Standard Name NPA3 1
Systematic Name YJR072C
Alias EPA1 2 , GPN1 3
Feature Type ORF, Verified
Description Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description Nucleolar Preribosomal Associated 1
Chromosomal Location
ChrX:572124 to 570967 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All NPA3 GO evidence and references
  View Computational GO annotations for NPA3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
High-throughput
Regulators 3 genes
Resources
Classical genetics
overexpression
repressible
Large-scale survey
conditional
null
reduction of function
Resources
93 total interaction(s) for 64 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 70
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 5
  • Biochemical Activity: 6
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Synthetic Lethality: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 385
Molecular Weight (Da) 43,194
Isoelectric Point (pI) 4.58
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:572124 to 570967 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1158 572124..570967 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003833
References cited on this page View Complete Literature Guide for NPA3
1) Dez C, et al.  (2004) Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification. Mol Cell Biol 24(14):6324-37
2) Keniry ME, et al.  (2004) The identification of Pcl1-interacting proteins that genetically interact with Cla4 may indicate a link between G1 progression and mitotic exit. Genetics 166(3):1177-86
3) Alonso B, et al.  (2011) A role for GPN-loop GTPase yGPN1 in sister chromatid cohesion. Cell Cycle 10(11):1828-37
4) Staresincic L, et al.  (2011) GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein. J Biol Chem 286(41):35553-61
5) Gras S, et al.  (2007) Structural insights into a new homodimeric self-activated GTPase family. EMBO Rep 8(6):569-75
6) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76