RFC2/YJR068W Summary

RFC2 BASIC INFORMATION

Standard Name	RFC2 1
Systematic Name	YJR068W
Feature Type	ORF, Verified
Description	Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon (2, 3)
Name Description	Replication Factor C 2

GO Annotations	All RFC2 GO evidence and references
	View Computational GO annotations for RFC2
Molecular Function
Manually curated	contributes_to DNA clamp loader activity (IDA) purine nucleotide binding (ISS)
Biological Process
Manually curated	cell cycle checkpoint (IMP) leading strand elongation (IDA) mismatch repair (TAS) sister chromatid cohesion (IPI)
Cellular Component
Manually curated	Ctf18 RFC-like complex (IPI) DNA replication factor C complex (IDA) Elg1 RFC-like complex (IPI) Rad17 RFC-like complex (IDA)

Mutant Phenotype	All RFC2 Phenotype details and references
Classical genetics
conditional	UV resistance: decreased cell cycle progression in S phase: abnormal chromosome/plasmid maintenance: abnormal heat sensitivity: increased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased spindle morphology: abnormal
reduction of function	UV resistance: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased vegetative growth: decreased rate
Large-scale survey
null	inviable resistance to sulfanilamide: decreased
repressible	cell cycle progression in G2 phase: abnormal

Interactions	RFC2 All interactions details and references
	87 total interaction(s) for 34 unique genes/features.
Physical Interactions	Affinity Capture-MS: 33 Affinity Capture-RNA: 1 Affinity Capture-Western: 15 Co-purification: 18 PCA: 1 Reconstituted Complex: 7 Two-hybrid: 4
Genetic Interactions	Dosage Rescue: 1 Synthetic Lethality: 6 Synthetic Rescue: 1

Sequence Information

ChrX:567635 to 568696 | |

Last Update

Coordinates: 2009-02-18 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1062	567635..568696	2009-02-18	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003829

RFC2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for RFC2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RFC2]

1)	Noskov V, et al. (1994) The RFC2 gene encoding a subunit of replication factor C of Saccharomyces cerevisiae. Nucleic Acids Res 22(9):1527-35
2)	Cullmann G, et al. (1995) Characterization of the five replication factor C genes of Saccharomyces cerevisiae. Mol Cell Biol 15(9):4661-71
3)	Yao N, et al. (2003) Replication factor C clamp loader subunit arrangement within the circular pentamer and its attachment points to proliferating cell nuclear antigen. J Biol Chem 278(50):50744-53

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