RPA12/YJR063W Summary

RPA12 BASIC INFORMATION

Standard Name	RPA12 1
Systematic Name	YJR063W
Alias	RRN4 2
Feature Type	ORF, Verified
Description	RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex (1, 3)
Name Description	RNA Polymerase A
Gene Product Alias	A12.2 4

GO Annotations	All RPA12 GO evidence and references
	View Computational GO annotations for RPA12
Molecular Function
Manually curated	exoribonuclease activity (IMP)
Biological Process
Manually curated	termination of RNA polymerase I transcription (IMP) transcription of nuclear rRNA large RNA polymerase I transcript (IGI)
Cellular Component
Manually curated	DNA-directed RNA polymerase I complex (IDA)

Mutant Phenotype	All RPA12 Phenotype details and references
Classical genetics
conditional	rRNA species transcribed by RNA polymerase I accumulation: decreased heat sensitivity: increased
null	heat sensitivity: increased RNA polymerase I subunits accumulation: decreased
Large-scale survey
null	glycogen accumulation: decreased competitive fitness: decreased fermentative growth: decreased rate heat sensitivity: increased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to ethanol: decreased resistance to methanol: decreased resistance to propan-1-ol: decreased viable

Interactions	RPA12 All interactions details and references
	65 total interaction(s) for 22 unique genes/features.
Physical Interactions	Affinity Capture-MS: 48 Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Biochemical Activity: 3 Two-hybrid: 3
Genetic Interactions	Dosage Rescue: 1 Synthetic Growth Defect: 3 Synthetic Lethality: 4

Sequence Information

ChrX:555187 to 555564 | |

Last Update

Coordinates: 2009-02-18 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..378	555187..555564	2009-02-18	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003824

RPA12 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for RPA12

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for RPA12]

1)	Nogi Y, et al. (1993) Gene RRN4 in Saccharomyces cerevisiae encodes the A12.2 subunit of RNA polymerase I and is essential only at high temperatures. Mol Cell Biol 13(1):114-22
2)	Nogi Y, et al. (1991) An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 88(16):7026-30
3)	Van Mullem V, et al. (2002) Rpa12p, a conserved RNA polymerase I subunit with two functional domains. Mol Microbiol 43(5):1105-13
4)	Riva M, et al. (1982) Natural variation in yeast RNA polymerase A. Formation of a mosaic RNA polymerase A in a meiotic segregant from an interspecific hybrid. J Biol Chem 257(8):4570-7

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