CBF1/YJR060W Summary Help

CBF1 BASIC INFORMATION

Standard Name CBF1
Systematic Name YJR060W
Alias CEP1 , CPF1
Feature Type ORF, Verified
Description Helix-loop-helix protein that binds the motif CACRTG, which is present at several sites including MET gene promoters and centromere DNA element I (CDEI); required for nucleosome positioning at this motif; targets Isw1p to DNA (1, 2, 3)
Name Description Centromere-Binding Factor 4
GO Annotations All CBF1 GO evidence and references
    View Computational GO annotations for CBF1
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
High-throughput
Cellular Component
Manually curated
High-throughput
Regulatory Role
Binding motifs CBF1 Transcription Factor Binding Sites and References
  • TCACGTG
Regulatory modules predicted: stressResponse ( 487 , 361 )
predicted: cellcycle ( 359 , 311 )
predicted: stressResponse ( 487 , 361 )
predicted: cellcycle ( 359 , 311 )
Mutant Phenotype All CBF1 Phenotype details and references
Classical genetics
overexpression
Large-scale survey
null
overexpression
Interactions CBF1 All interactions details and references
58 total interaction(s) for 43 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-Western: 2
  • Biochemical Activity: 1
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 6
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 10
  • Synthetic Lethality: 21
  • Synthetic Rescue: 2
Sequence Information
ChrX:548751 to 549806 | ORF Map | GBrowse
Gbrowse
Genetic position: 40 cM
Last Update Coordinates: 2009-02-18 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1056 548751..549806 2009-02-18 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003821

CBF1 RESOURCES

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SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for CBF1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for CBF1]

1) Wieland G, et al.  (2001) Determination of the binding constants of the centromere protein Cbf1 to all 16 centromere DNAs of Saccharomyces cerevisiae. Nucleic Acids Res 29(5):1054-60
2) Moreau JL, et al.  (2003) Regulated displacement of TBP from the PHO8 promoter in vivo requires Cbf1 and the Isw1 chromatin remodeling complex. Mol Cell 11(6):1609-20
3) Kent NA, et al.  (2004) Cbf1p is required for chromatin remodeling at promoter-proximal CACGTG motifs in yeast. J Biol Chem 279(26):27116-23
4) Thomas D, et al.  (1992) MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae. Mol Cell Biol 12(4):1719-27