NUP85/YJR042W Summary Help

NUP85 BASIC INFORMATION

Standard Name NUP85
Systematic Name YJR042W
Alias RAT9
Feature Type ORF, Verified
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), required for assembly of the subcomplex and also for formation of the nucleocytoplasmic Gsp1p concentration gradient that plays a role in nuclear trafficking (1, 2 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP85 GO evidence and references
    View Computational GO annotations for NUP85
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All NUP85 Phenotype details and references
Classical genetics
conditional
Large-scale survey
null
Interactions NUP85 All interactions details and references
98 total interaction(s) for 30 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 46
  • Affinity Capture-Western: 10
  • Co-crystal Structure: 2
  • Co-localization: 1
  • Co-purification: 7
  • FRET: 3
  • PCA: 3
  • Reconstituted Complex: 8
  • Two-hybrid: 1
Genetic Interactions
  • Dosage Rescue: 1
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 9
  • Synthetic Rescue: 2
Sequence Information
ChrX:514047 to 516281 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2009-02-18 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2235 514047..516281 2009-02-18 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003803

NUP85 RESOURCES

Click on map for expanded view
SGD ORF mapGBrowse
SGD ORF map
 GBrowse
  • Literature
  • Retrieve Sequences
  • Sequence Analysis Tools
  • Protein Info & Structure
  • Localization Resources
  • Interactions
  • Phenotype Resources
  • Maps & Displays
  • Comparison Resources
  • Functional Analysis

Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for NUP85

SUMMARY PARAGRAPH for NUP85

NUP85 encodes a nuclear pore protein that was identified independently by screens for mutants defective in mRNA export from the nucleus (3) and for synthetic lethals with an nsp1 temperature sensitive mutation (4, 5). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 5, 6, 7, 8). The structure of the vertebrate NPC has been studied extensively; recent reviews include 9, 10, 11, and 12. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (13, 14). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 5, 6, 15, 4, 16). Nup85p is part of subcomplex within the NPC that also includes Sec13p, Seh1p, Nup84p, and Nup120p (17, 5). Nup85p is tightly associated with Seh1p in the subcomplex (17). While Nup85p is not essential, nup85 deletion strains are temperature sensitive and show defects in nuclear envelope morphology and NPC distribution (3, 17). The nup85 deletion is synthetically lethal with mutations in the mRNA export factors Mex67p and Mtr2p (18, 19), and Mtr2p interacts physically with Nup85p (19).

Last updated: 1999-08-04

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP85]

1) Siniossoglou S, et al.  (2000) Structure and assembly of the Nup84p complex. J Cell Biol 149(1):41-54
2) Gao H, et al.  (2003) Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p. J Biol Chem 278(28):25331-40
3) Goldstein AL, et al.  (1996) Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p. Mol Biol Cell 7(6):917-34
4) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
5) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
6) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
7) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
8) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
9) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
10) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
11) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
12) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
13) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
14) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
15) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
16) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
17) Siniossoglou S, et al.  (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell 84(2):265-75
18) Segref A, et al.  (1997) Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores. EMBO J 16(11):3256-71
19) Santos-Rosa H, et al.  (1998) Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores. Mol Cell Biol 18(11):6826-38