GEF1/YJR040W Summary Help

Standard Name GEF1 1
Systematic Name YJR040W
Alias CLC
Feature Type ORF, Verified
Description Voltage-gated chloride channel; localized to the golgi, the endosomal system, and plasma membrane; involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism (2, 3, 4, 5)
Name Description Glycerol Ethanol, Ferric requiring 1
Chromosomal Location
ChrX:507746 to 510085 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All GEF1 GO evidence and references
  View Computational GO annotations for GEF1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Classical genetics
null
unspecified
Large-scale survey
null
unspecified
Resources
105 total interaction(s) for 66 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 3
  • Affinity Capture-Western: 2
  • PCA: 6
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 72
  • Phenotypic Suppression: 1
  • Positive Genetic: 7
  • Synthetic Growth Defect: 6
  • Synthetic Lethality: 4
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 779
Molecular Weight (Da) 87,591
Isoelectric Point (pI) 7.07
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:507746 to 510085 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2340 507746..510085 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | TCDB | UniProtKB
Primary SGDIDS000003801
References cited on this page View Complete Literature Guide for GEF1
1) Greene JR, et al.  (1993) The GEF1 gene of Saccharomyces cerevisiae encodes an integral membrane protein; mutations in which have effects on respiration and iron-limited growth. Mol Gen Genet 241(5-6):542-53
2) Gaxiola RA, et al.  (1998) The yeast CLC chloride channel functions in cation homeostasis. Proc Natl Acad Sci U S A 95(7):4046-50
3) Flis K, et al.  (2002) The Gef1 protein of Saccharomyces cerevisiae is associated with chloride channel activity. Biochem Biophys Res Commun 294(5):1144-50
4) Lopez-Rodriguez A, et al.  (2007) The product of the gene GEF1 of Saccharomyces cerevisiae transports Cl across the plasma membrane. FEMS Yeast Res 7(8):1218-29
5) Sasvari Z, et al.  (2013) The GEF1 proton-chloride exchanger affects tombusvirus replication via regulation of copper metabolism in yeast. J Virol 87(3):1800-10