RAV1/YJR033C Summary Help

Standard Name RAV1 1
Systematic Name YJR033C
Alias SOI3 2 , 3
Feature Type ORF, Verified
Description Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate (1, 2, 3, 4, 5)
Name Description Regulator of (H+)-ATPase in Vacuolar membrane 1
Chromosomal Location
ChrX:496451 to 492378 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All RAV1 GO evidence and references
  View Computational GO annotations for RAV1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Classical genetics
null
unspecified
Large-scale survey
null
Resources
113 total interaction(s) for 69 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 15
  • Affinity Capture-Western: 8
  • Biochemical Activity: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 7

Genetic Interactions
  • Negative Genetic: 44
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 10
  • Positive Genetic: 3
  • Synthetic Growth Defect: 7
  • Synthetic Lethality: 3
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,357
Molecular Weight (Da) 154,932
Isoelectric Point (pI) 8.2
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:496451 to 492378 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..4074 496451..492378 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003794
References cited on this page View Complete Literature Guide for RAV1
1) Seol JH, et al.  (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91
2) Redding K, et al.  (1996) Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins. Mol Cell Biol 16(11):6208-17
3) Sipos G, et al.  (2004) Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins. Mol Biol Cell 15(7):3196-209
4) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5) Smardon AM, et al.  (2002) The RAVE complex is essential for stable assembly of the yeast V-ATPase. J Biol Chem 277(16):13831-9