ERG20/YJL167W Summary

Standard Name	ERG20 1
Systematic Name	YJL167W
Alias	BOT3 , FDS1 , FPP1
Feature Type	ORF, Verified
Description	Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis (2, 3 and see Summary Paragraph)
Name Description	ERGosterol biosynthesis 1

Chromosomal Location	ChrX:105014 to 106072 \| ORF Map \| GBrowse

Gene Ontology Annotations All ERG20 GO evidence and references

	View Computational GO annotations for ERG20
Molecular Function
Manually curated	dimethylallyltranstransferase activity (IDA) geranyltranstransferase activity (IDA)
Biological Process
Manually curated	ergosterol biosynthetic process (IMP) farnesyl diphosphate biosynthetic process (IDA) isoprenoid biosynthetic process (IDA)
Cellular Component
Manually curated	cytoplasm (IDA) endoplasmic reticulum (IDA)

Pathways	ergosterol biosynthesis hexaprenyl diphosphate biosynthesis superpathway of ergosterol biosynthesis superpathway of ubiquinone biosynthesis

Mutant phenotypes All ERG20 Phenotype evidence and references

Classical genetics
null	inviable
Large-scale survey
conditional	colony sectoring: increased
null	inviable
overexpression	vegetative growth: decreased rate
reduction of function	competitive fitness: decreased resistance to hydroxyurea: decreased resistance to methyl methanesulfonate: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All ERG20 Interaction evidence and references

	46 total interaction(s) for 41 unique genes/features.
Physical Interactions	Affinity Capture-MS: 12 Affinity Capture-RNA: 1 Biochemical Activity: 3 Co-fractionation: 1 Protein-peptide: 2 Reconstituted Complex: 1 Two-hybrid: 5
Genetic Interactions	Dosage Rescue: 1 Negative Genetic: 15 Phenotypic Enhancement: 3 Positive Genetic: 2
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All ERG20 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| AspGD Homologs \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrX:105014 to 106072 | |

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..1059	105014..106072	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003703

SUMMARY PARAGRAPH for ERG20

ERG20 encodes farnesyl pyrophosphate synthetase (FPP synthase), a cytosolic enzyme that has dimethylallyltranstransferase and geranyltranstransferase activities (3, 2, 4). FPP synthase catalyzes the sequential 1'-4 coupling of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate and geranyl diphosphate, forming C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis; in yeast ergosterol is the end product of the sterol biosynthetic pathway (3, 5, 4).

ERG20 is essential; erg20 mutations cause ergosterol auxotrophy, and erg20 mutant cells excrete prenyl alcohols (3, 6). FPP synthases have been identified in many organisms (3, 7, 4); the Arabidopsis gene FPS1 can complement the yeast erg20 null phenotype (8).

Last updated: 2000-08-28

References cited on this page View Complete Literature Guide for ERG20

1)	Chambon C, et al. (1991) Sterol pathway in yeast. Identification and properties of mutant strains defective in mevalonate diphosphate decarboxylase and farnesyl diphosphate synthetase. Lipids 26(8):633-6
2)	Parks LW, et al. (1995) Biochemical and physiological effects of sterol alterations in yeast--a review. Lipids 30(3):227-30
3)	Anderson MS, et al. (1989) Farnesyl diphosphate synthetase. Molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiae. J Biol Chem 264(32):19176-84
4)	Stryer L (1995) Biochemistry (4th ed.). New York: W. H. Freeman and Company
5)	Paltauf F, et al. (1992) "Regulation and compartmentalization of lipid synthesis in yeast." Pp. 415-500 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6)	Chambon C, et al. (1990) Isolation and properties of yeast mutants affected in farnesyl diphosphate synthetase. Curr Genet 18(1):41-6
7)	Ohnuma S, et al. (1997) Conversion from archaeal geranylgeranyl diphosphate synthase to farnesyl diphosphate synthase. Two amino acids before the first aspartate-rich motif solely determine eukaryotic farnesyl diphosphate synthase activity. J Biol Chem 272(8):5192-8
8)	Cunillera N, et al. (1996) Arabidopsis thaliana contains two differentially expressed farnesyl-diphosphate synthase genes. J Biol Chem 271(13):7774-80