FAR1/YJL157C Summary 
| Standard Name | FAR1 1 |
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| Systematic Name | YJL157C |
| Feature Type | ORF, Verified |
| Description | Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress (1, 2, 3, 4, 5 and see Summary Paragraph) |
| Name Description | Factor ARrest 1 |
| Chromosomal Location | |
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| Note: this feature is encoded on the Crick strand. | |
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| View Computational GO annotations for FAR1 | |
| Molecular Function | |
| Manually curated | |
| Biological Process | |
| Manually curated | |
| Cellular Component | |
| Manually curated | |
| High-throughput |
| Regulatory modules | predicted: stressResponse (601, 493, 427) |
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| 132 total interaction(s) for 71 unique genes/features. | |
| Physical Interactions |
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| Genetic Interactions |
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| Localization | |
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| Phosphorylation | PhosphoGRID | PhosphoPep Database |
| Structure | |
| Homologs |
| Note: this feature is encoded on the Crick strand. | |||||||||||||
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| Last Update | Coordinates: 2011-02-03 | Sequence: 1996-07-31 | ||||||||||||
| Subfeature details |
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| S288C only | |
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| S288C vs. other species | |
| S288C vs. other strains |
| External Links | All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB |
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| Primary SGDID | S000003693 |
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The cyclin dependent kinase inhibitor Far1p is able to inactivate Cdc28p/Clnp complexes, and thus helps stop the cell cycle at START in G1 (2, 6). This is important for mating, in which haploid cells of opposite mating type synchronize their cell cycles by arresting at START, so that they can fuse and become a diploid. Transcription of FAR1 is induced by mating pheromone, in a Ste12p-dependent manner (1). Far1p levels are also controlled by ubiquitin-mediated proteolysis; Far1p is unstable throughout the cell cycle except during G1 (4). Phosphorylation by the MAP kinase homolog Fus3p may help regulate Far1p's association with the Cdc28p/Cln2p complex (7). Far1p may also serve another function in the cell, as part of a Cdc24p-G/beta/gamma-Far1p complex that acts as a landmark for oriented growth during mating (3, 8). This would be made possible by the movement of Far1p from the nucleus to the cytoplasm in response to mating pheromone (8).
Last updated: 1999-09-01 
| 1) | Chang F and Herskowitz I (1990) Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2. Cell 63(5):999-1011 |
| 2) | Peter M and Herskowitz I (1994) Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1. Science 265(5176):1228-31 |
| 3) | Nern A and Arkowitz RA (1999) A Cdc24p-Far1p-Gbetagamma protein complex required for yeast orientation during mating. J Cell Biol 144(6):1187-202 |
| 4) | Henchoz S, et al. (1997) Phosphorylation- and ubiquitin-dependent degradation of the cyclin-dependent kinase inhibitor Far1p in budding yeast. Genes Dev 11(22):3046-60 |
| 5) | Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76 |
| 6) | Mendenhall MD (1998) Cyclin-dependent kinase inhibitors of Saccharomyces cerevisiae and Schizosaccharomyces pombe. Curr Top Microbiol Immunol 227():1-24 |
| 7) | Peter M, et al. (1993) FAR1 links the signal transduction pathway to the cell cycle machinery in yeast. Cell 73(4):747-60 |
| 8) | Butty AC, et al. (1998) The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating. Science 282(5393):1511-6 |
