TIF2/YJL138C Summary Help

Standard Name TIF2 1
Systematic Name YJL138C
Feature Type ORF, Verified
Description Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7)
Name Description Translation Initiation Factor
Gene Product Alias eIF4A
Chromosomal Location
ChrX:154691 to 153504 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Genetic position: -92 cM
Gene Ontology Annotations All TIF2 GO evidence and references
  View Computational GO annotations for TIF2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Mutant phenotypes All TIF2 Phenotype evidence and references
Classical genetics
null
Large-scale survey
null
overexpression
Resources
interactions All TIF2 Interaction evidence and references
187 total interaction(s) for 155 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 79
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 3
  • Co-crystal Structure: 1
  • Reconstituted Complex: 11
  • Two-hybrid: 1
Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 3
  • Negative Genetic: 60
  • Phenotypic Suppression: 2
  • Positive Genetic: 13
  • Synthetic Growth Defect: 5
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1
Resources
Expression Summary
histogram
Resources
Protein Information All TIF2 Protein evidence and references
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:154691 to 153504 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Genetic position: -92 cM
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..1188 154691..153504 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003674
References cited on this page View Complete Literature Guide for TIF2
1) Mueller, P. and Linder, P.  (1989) Personal Communication, Mortimer Map Edition 10
2) Blum S, et al.  (1989) Translation in Saccharomyces cerevisiae: initiation factor 4A-dependent cell-free system. Proc Natl Acad Sci U S A 86(16):6043-6
3) Neff CL and Sachs AB  (1999) Eukaryotic translation initiation factors 4G and 4A from Saccharomyces cerevisiae interact physically and functionally. Mol Cell Biol 19(8):5557-64
4) Chang TH, et al.  (1990) Identification of five putative yeast RNA helicase genes. Proc Natl Acad Sci U S A 87(4):1571-5
5) Caruthers JM, et al.  (2000) Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase. Proc Natl Acad Sci U S A 97(24):13080-5
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76