SRS2/YJL092W Summary

SRS2/YJL092W Summary

Standard Name	SRS2 1
Systematic Name	YJL092W
Alias	HPR5 2
Feature Type	ORF, Verified
Description	DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability (3, 4, 5, 6, 7, 8, 9, 10) Also known as: RADH 2 , RADH1
Name Description	Suppressor of Rad Six 1

Chromosomal Location	ChrX:257423 to 260947 \| ORF Map \| GBrowse

	Genetic position: -61 cM

Gene Ontology Annotations All SRS2 GO evidence and references

	View Computational GO annotations for SRS2
Molecular Function
Manually curated	DNA helicase activity (IDA, ISS)
Biological Process
Manually curated	DNA duplex unwinding (IDA) DNA repair (IGI) double-strand break repair via nonhomologous end joining (IDA)
Cellular Component
Manually curated	nucleus (IDA)

Mutant phenotypes All SRS2 Phenotype evidence and references

Classical genetics
null	UV resistance: decreased bud morphology: abnormal cell cycle passage through the metaphase-anaphase transition: arrested chromosome/plasmid maintenance: abnormal gamma ray resistance: decreased lifespan: decreased meiotic recombination: delayed mitotic recombination: increased resistance to 4-aminofolic acid: decreased spore germination: decreased viable
overexpression	cell cycle progression in S phase: delayed Rad53p modification: increased
Large-scale survey
null	UV resistance: decreased competitive fitness: decreased haploinsufficient heat sensitivity: increased resistance to fluconazole: increased resistance to sirolimus: increased resistance to hydroxyurea: decreased resistance to 5-fluorouracil: decreased resistance to methyl methanesulfonate: decreased resistance to camptothecin: decreased resistance to sodium selenide: decreased resistance to cisplatin: decreased resistance to doxorubicin: decreased transposable element transposition: increased viable
overexpression	vegetative growth: decreased rate
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SRS2 Interaction evidence and references

	915 total interaction(s) for 460 unique genes/features.
Physical Interactions	Affinity Capture-MS: 14 Affinity Capture-RNA: 2 Affinity Capture-Western: 9 Biochemical Activity: 4 Co-crystal Structure: 2 PCA: 1 Reconstituted Complex: 15 Two-hybrid: 95
Genetic Interactions	Dosage Lethality: 257 Dosage Rescue: 7 Negative Genetic: 113 Phenotypic Enhancement: 19 Phenotypic Suppression: 22 Positive Genetic: 15 Synthetic Growth Defect: 159 Synthetic Lethality: 106 Synthetic Rescue: 75
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SRS2 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrX:257423 to 260947 | |

SGD ORF map

: -61 cM

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..3525	257423..260947	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003628

References cited on this page View Complete Literature Guide for SRS2

1)	Lawrence CW and Christensen RB (1979) Metabolic suppressors of trimethoprim and ultraviolet light sensitivities of Saccharomyces cerevisiae rad6 mutants. J Bacteriol 139(3):866-76
2)	Rong L, et al. (1991) The hyper-gene conversion hpr5-1 mutation of Saccharomyces cerevisiae is an allele of the SRS2/RADH gene. Genetics 127(1):75-85
3)	Palladino F and Klein HL (1992) Analysis of mitotic and meiotic defects in Saccharomyces cerevisiae SRS2 DNA helicase mutants. Genetics 132(1):23-37
4)	Aboussekhra A, et al. (1989) RADH, a gene of Saccharomyces cerevisiae encoding a putative DNA helicase involved in DNA repair. Characteristics of radH mutants and sequence of the gene. Nucleic Acids Res 17(18):7211-9
5)	Rong L and Klein HL (1993) Purification and characterization of the SRS2 DNA helicase of the yeast Saccharomyces cerevisiae. J Biol Chem 268(2):1252-9
6)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
7)	Pfander B, et al. (2005) SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase. Nature 436(7049):428-33
8)	Papouli E, et al. (2005) Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p. Mol Cell 19(1):123-33
9)	Dhar A and Lahue RS (2008) Rapid unwinding of triplet repeat hairpins by Srs2 helicase of Saccharomyces cerevisiae. Nucleic Acids Res 36(10):3366-73
10)	Yeung M and Durocher D (2011) Srs2 enables checkpoint recovery by promoting disassembly of DNA damage foci from chromatin. DNA Repair (Amst) 10(12):1213-22