SIP4/YJL089W Summary Help

Standard Name SIP4 1, 2
Systematic Name YJL089W
Feature Type ORF, Verified
Description C6 zinc cluster transcriptional activator; binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus (1, 3, 4)
Name Description SNF1-Interacting Protein 2
Chromosomal Location
ChrX:265926 to 268415 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All SIP4 GO evidence and references
  View Computational GO annotations for SIP4
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Targets 10 genes
Regulators 8 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
83 total interaction(s) for 64 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 2
  • Biochemical Activity: 6
  • Reconstituted Complex: 1
  • Two-hybrid: 10

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 54
  • Positive Genetic: 5

Resources
Expression Summary
histogram
Resources
Length (a.a.) 829
Molecular Weight (Da) 96,004
Isoelectric Point (pI) 6.51
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:265926 to 268415 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2490 265926..268415 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003625
References cited on this page View Complete Literature Guide for SIP4
1) Lesage P, et al.  (1996) Yeast SNF1 protein kinase interacts with SIP4, a C6 zinc cluster transcriptional activator: a new role for SNF1 in the glucose response. Mol Cell Biol 16(5):1921-8
2) Yang X, et al.  (1992) A protein kinase substrate identified by the two-hybrid system. Science 257(5070):680-2
3) Vincent O and Carlson M  (1998) Sip4, a Snf1 kinase-dependent transcriptional activator, binds to the carbon source-responsive element of gluconeogenic genes. EMBO J 17(23):7002-8
4) Roth S, et al.  (2004) Transcriptional activators Cat8 and Sip4 discriminate between sequence variants of the carbon source-responsive promoter element in the yeast Saccharomyces cerevisiae. Curr Genet 45(3):121-8
5) Harbison CT, et al.  (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
6) Zhu C, et al.  (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
7) Badis G, et al.  (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87