SIP4/YJL089W Summary

SIP4/YJL089W Summary

Standard Name	SIP4 1, 2
Systematic Name	YJL089W
Feature Type	ORF, Verified
Description	C6 zinc cluster transcriptional activator that binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus (1, 3, 4)
Name Description	SNF1-Interacting Protein 2

Chromosomal Location	ChrX:265926 to 268415 \| ORF Map \| GBrowse

Gene Ontology Annotations All SIP4 GO evidence and references

	View Computational GO annotations for SIP4
Molecular Function
Manually curated	RNA polymerase II core promoter proximal region sequence-specific DNA binding (IDA) RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription (IDA)
High-throughput	sequence-specific DNA binding (IDA)
Biological Process
Manually curated	negative regulation of invasive growth in response to glucose limitation (IMP) positive regulation of gluconeogenesis (IPI) positive regulation of gluconeogenesis by positive regulation of transcription from RNA polymerase II promoter (IMP) positive regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source (IGI, IMP)
Cellular Component
Manually curated	nucleus (IDA)

Regulatory Role

Binding motifs	Predicted SIP4 Binding Site Locations
	CGGNYNAATGGRR 5 [Search intergenic regions for CGGNYNAATGGRR] TCCATTSRTCCGR 4 [Search intergenic regions for TCCATTSRTCCGR] TCCGGA 6 [Search intergenic regions for TCCGGA] YYCGGRR 7 [Search intergenic regions for YYCGGRR]

Regulatory modules	predicted: stressResponse (487)

Resources	ScerTF \| UniProbe \| YEASTRACT \| YeTFaSCo

Mutant phenotypes All SIP4 Phenotype evidence and references

Classical genetics
null	dessication resistance: decreased viable
Large-scale survey
null	budding index: decreased competitive fitness: normal resistance to chloramphenicol: normal resistance to 4-nitroquinoline N-oxide: normal resistance to oligomycin: normal resistance to rhodamine 6-G: normal resistance to cycloheximide: normal resistance to ketoconazole: normal viable
overexpression	vegetative growth: decreased rate vegetative growth: decreased
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All SIP4 Interaction evidence and references

	82 total interaction(s) for 63 unique genes/features.
Physical Interactions	Affinity Capture-RNA: 1 Affinity Capture-Western: 2 Biochemical Activity: 6 Reconstituted Complex: 1 Two-hybrid: 10
Genetic Interactions	Dosage Growth Defect: 1 Dosage Lethality: 1 Dosage Rescue: 1 Negative Genetic: 54 Positive Genetic: 5
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All SIP4 Protein evidence and references

Localization	YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrX:265926 to 268415 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2490	265926..268415	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003625

References cited on this page View Complete Literature Guide for SIP4

1)	Lesage P, et al. (1996) Yeast SNF1 protein kinase interacts with SIP4, a C6 zinc cluster transcriptional activator: a new role for SNF1 in the glucose response. Mol Cell Biol 16(5):1921-8
2)	Yang X, et al. (1992) A protein kinase substrate identified by the two-hybrid system. Science 257(5070):680-2
3)	Vincent O and Carlson M (1998) Sip4, a Snf1 kinase-dependent transcriptional activator, binds to the carbon source-responsive element of gluconeogenic genes. EMBO J 17(23):7002-8
4)	Roth S, et al. (2004) Transcriptional activators Cat8 and Sip4 discriminate between sequence variants of the carbon source-responsive promoter element in the yeast Saccharomyces cerevisiae. Curr Genet 45(3):121-8
5)	Harbison CT, et al. (2004) Transcriptional regulatory code of a eukaryotic genome. Nature 431(7004):99-104
6)	Zhu C, et al. (2009) High-resolution DNA-binding specificity analysis of yeast transcription factors. Genome Res 19(4):556-66
7)	Badis G, et al. (2008) A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters. Mol Cell 32(6):878-87