EXO70/YJL085W Summary Help

Standard Name EXO70 1
Systematic Name YJL085W
Feature Type ORF, Verified
Description Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Name Description EXOcyst 1
Chromosomal Location
ChrX:272827 to 274698 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All EXO70 GO evidence and references
  View Computational GO annotations for EXO70
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
conditional
dominant negative
reduction of function
Large-scale survey
null
reduction of function
Resources
157 total interaction(s) for 87 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 19
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 22
  • Co-crystal Structure: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Rescue: 14
  • Negative Genetic: 57
  • Phenotypic Enhancement: 2
  • Positive Genetic: 6
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 11
  • Synthetic Rescue: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 623
Molecular Weight (Da) 71,293
Isoelectric Point (pI) 7.52
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:272827 to 274698 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1872 272827..274698 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003621
References cited on this page View Complete Literature Guide for EXO70
1) TerBush DR, et al.  (1996) The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J 15(23):6483-94
2) Adamo JE, et al.  (1999) The Rho GTPase Rho3 has a direct role in exocytosis that is distinct from its role in actin polarity. Mol Biol Cell 10(12):4121-33
3) Robinson NG, et al.  (1999) Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70. Mol Cell Biol 19(5):3580-7
4) He B, et al.  (2007) Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. EMBO J 26(18):4053-65
5) Hutagalung AH, et al.  (2009) An internal domain of Exo70p is required for actin-independent localization and mediates assembly of specific exocyst components. Mol Biol Cell 20(1):153-63
6) Wu H, et al.  (2010) The exo70 subunit of the exocyst is an effector for both cdc42 and rho3 function in polarized exocytosis. Mol Biol Cell 21(3):430-42
7) Tkach JM, et al.  (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76