SCP160/YJL080C Summary Help

Standard Name SCP160 1
Systematic Name YJL080C
Feature Type ORF, Verified
Description Essential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins (2, 3, 4, 5, 6 and see Summary Paragraph)
Name Description S. cerevisiae protein involved in the Control of Ploidy 7
Chromosomal Location
ChrX:289226 to 285558 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All SCP160 GO evidence and references
  View Computational GO annotations for SCP160
Molecular Function
Manually curated
High-throughput
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
95 total interaction(s) for 76 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 45
  • Affinity Capture-RNA: 14
  • Affinity Capture-Western: 13
  • Biochemical Activity: 3
  • Co-fractionation: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 3

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Resources
Expression Summary
histogram
Resources
Length (a.a.) 1,222
Molecular Weight (Da) 134,808
Isoelectric Point (pI) 5.67
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:289226 to 285558 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..3669 289226..285558 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003616
SUMMARY PARAGRAPH for SCP160

Scp160p is an RNA-binding protein containing 14 tandemly-repeated heterogenous nuclear ribonucleoprotein K-homology (KH) domains, a potential nuclear-export signal (NES) near its N-terminus, and a potential nuclear-localization signal (NLS) between KH domains 3 and 4 (8, 7). The protein overall demonstrates significant sequence homology to a family of vertebrate RNA-binding proteins collectively known as vigilins (2).

Scp160p interacts with free and membrane-bound polyribosomes that are dependent upon the presence of specific mRNAs and Bfr1p (8, 9). Scp160p associates with specific (as opposed to random) messages, among which are mRNAs derived from the BIK1 (microtubule-associated protein), DHH1 (helicase), NAM8 (snRNP component), YOR338W, and YOL155C (glucosidase) genes (10). Despite its presence on cytosolic polyribosomes, Scp160p is predominantly associated with the nuclear envelope and the endoplasmic reticulum (ER) (7). Accumulation of Scp160p-ribosome complexes at the ER requires the function of microtubules but is independent of the actin cytoskeleton (8).

Scp160p is in close proximity to translation elongation factor 1A and the WD40 (Trp-Asp 40)-repeat containing protein Asc1p at ribosomes. The C-terminus of Scp160p is essential for ribosome binding, and this interaction depends on Asc1p. It has been suggested that Scp160p connects specific mRNAs, ribosomes, and a translation factor with an adaptor for signalling molecules. These interactions might regulate the translation activity of ribosomes programmed with specific mRNAs (4).

Scp160p is also an essential component of the mating response pathway and is the first RNA-binding protein to be indentified as a G protein effector. Scp160p binds the Gpa1p GTPase and signaling by activated Gpa1p requires this direct coupling to Scp160p (3).

SCP160 interacts genetically and biochemically with EAP1, whose protein product functions in translation as an eIF4E-binding protein with additional uncharacterized spindle pole body functions, confirming that Scp160p plays a role in translation. Loss of either gene results in significant changes in either the complex associations or subcellular distribution of the other protein (11). Disruption of SCP160 also results in decreased viability, abnormal morphology, and increased DNA content, a complex phenotype that is not reversible by transformation with a plasmid carrying the wild-type gene (1). Further, loss of Scp160p results in changes in both the abundance and distribution between soluble and membrane-associated fractions for some messages (YOR338W), and in a subtle shift from soluble polyribosomes to soluble mRNPs for others (DHH1 and YOR338W) (10).

Last updated: 2005-03-30 Contact SGD

References cited on this page View Complete Literature Guide for SCP160
1) Wintersberger U, et al.  (1995) Scp160p, a new yeast protein associated with the nuclear membrane and the endoplasmic reticulum, is necessary for maintenance of exact ploidy. Yeast 11(10):929-44
2) Lang BD and Fridovich-Keil JL  (2000) Scp160p, a multiple KH-domain protein, is a component of mRNP complexes in yeast. Nucleic Acids Res 28(7):1576-84
3) Guo M, et al.  (2003) The yeast G protein alpha subunit Gpa1 transmits a signal through an RNA binding effector protein Scp160. Mol Cell 12(2):517-24
4) Baum S, et al.  (2004) Asc1p, a WD40-domain containing adaptor protein, is required for the interaction of the RNA-binding protein Scp160p with polysomes. Biochem J 380(Pt 3):823-30
5) Cortes A, et al.  (2003) Analysing the contribution of nucleic acids to the structure and properties of centric heterochromatin. Genetica 117(2-3):117-25
6) Gelin-Licht R, et al.  (2012) Scp160-dependent mRNA trafficking mediates pheromone gradient sensing and chemotropism in yeast. Cell Rep 1(5):483-94
7) Weber V, et al.  (1997) Purification and nucleic-acid-binding properties of a Saccharomyces cerevisiae protein involved in the control of ploidy. Eur J Biochem 249(1):309-17
8) Frey S, et al.  (2001) Scp160p, an RNA-binding, polysome-associated protein, localizes to the endoplasmic reticulum of Saccharomyces cerevisiae in a microtubule-dependent manner. J Biol Chem 276(19):15905-12
9) Lang BD, et al.  (2001) The brefeldin A resistance protein Bfr1p is a component of polyribosome-associated mRNP complexes in yeast. Nucleic Acids Res 29(12):2567-74
10) Li AM, et al.  (2003) Scp160p associates with specific mRNAs in yeast. Nucleic Acids Res 31(7):1830-7
11) Mendelsohn BA, et al.  (2003) Genetic and biochemical interactions between SCP160 and EAP1 in yeast. Nucleic Acids Res 31(20):5838-47