NET1/YJL076W Summary Help

Standard Name NET1 1, 2
Systematic Name YJL076W
Alias ESC5 2 , CFI1 3 , SRM8 4
Feature Type ORF, Verified
Description Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication (1, 2, 5, 6)
Name Description Nucleolar silencing Establishing factor and Telophase regulator 1, 2
Chromosomal Location
ChrX:295245 to 298814 | ORF Map | GBrowse
Gene Ontology Annotations All NET1 GO evidence and references
  View Computational GO annotations for NET1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 12 genes
Classical genetics
Large-scale survey
reduction of function
161 total interaction(s) for 85 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 74
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 29
  • Biochemical Activity: 7
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 4
  • Reconstituted Complex: 7
  • Two-hybrid: 12

Genetic Interactions
  • Dosage Growth Defect: 1
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 4
  • Synthetic Growth Defect: 3
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 1
  • Synthetic Rescue: 10

Expression Summary
Length (a.a.) 1,189
Molecular Weight (Da) 128,530
Isoelectric Point (pI) 8.09
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:295245 to 298814 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..3570 295245..298814 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003612
References cited on this page View Complete Literature Guide for NET1
1) Straight AF, et al.  (1999) Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity. Cell 97(2):245-56
2) Shou W, et al.  (1999) Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex. Cell 97(2):233-44
3) Visintin R, et al.  (1999) Cfi1 prevents premature exit from mitosis by anchoring Cdc14 phosphatase in the nucleolus. Nature 398(6730):818-23
4) Devin AB, et al.  (1994) [Isolation and characterization of new nuclear srm gene mutation causing coordinated changes in the maintenance of nuclear and mitochondrial genetic structures in the yeast Saccharomyces] Genetika 30(9):1194-201
5) Shou W, et al.  (2001) Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit. Mol Cell 8(1):45-55
6) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61