ARG2/YJL071W Summary Help

Standard Name ARG2
Systematic Name YJL071W
Alias HRB574
Feature Type ORF, Verified
Description Acetylglutamate synthase (glutamate N-acetyltransferase); mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p (1 and see Summary Paragraph)
Name Description ARGinine requiring
Chromosomal Location
ChrX:306132 to 307856 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All ARG2 GO evidence and references
  View Computational GO annotations for ARG2
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 5 genes
Resources
Pathways
Classical genetics
null
overexpression
Large-scale survey
null
Resources
23 total interaction(s) for 15 unique genes/features.
Physical Interactions
  • Affinity Capture-RNA: 1

Genetic Interactions
  • Phenotypic Enhancement: 17
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 4

Resources
Expression Summary
histogram
Resources
Length (a.a.) 574
Molecular Weight (Da) 65,609
Isoelectric Point (pI) 9.53
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:306132 to 307856 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..1725 306132..307856 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003607
SUMMARY PARAGRAPH for ARG2

ARG2 encodes glutamate N-acetyltransferase (also called acetylglutamate synthase), which catalyzes the first step in the biosynthesis of ornithine, an intermediate in arginine biosynthesis (2). Like other ornithine biosynthetic enzymes, Arg2p is localized in the mitochondrial matrix (2). ARG2 transcription is repressed in the presence of arginine and is also regulated by glucose repression and general amino acid control (3, 4). Arginine-responsive transcription factors, including Arg80p, Arg81p, Arg82p, and Mcm1p, and their target upstream activating sequences in ARG2, have been identified (5, 6, 4).

Last updated: 1999-10-15 Contact SGD

References cited on this page View Complete Literature Guide for ARG2
1) Abadjieva A, et al.  (2001) A new yeast metabolon involving at least the two first enzymes of arginine biosynthesis: acetylglutamate synthase activity requires complex formation with acetylglutamate kinase. J Biol Chem 276(46):42869-80
2) Jauniaux JC, et al.  (1978) Arginine metabolism in Saccharomyces cerevisiae: subcellular localization of the enzymes. J Bacteriol 133(3):1096-1107
3) Wipe B and Leisinger T  (1979) Regulation of activity and synthesis of N-acetylglutamate synthase from Saccharomyces cerevisiae. J Bacteriol 140(3):874-80
4) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
5) Messenguy F  (1987) Multiplicity of regulatory mechanisms controlling amino acid biosynthesis in Saccharomyces cerevisiae. Microbiol Sci 4(5):150-3
6) Messenguy F and Dubois E  (1993) Genetic evidence for a role for MCM1 in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol Cell Biol 13(4):2586-92