NUP82/YJL061W Summary Help

NUP82 BASIC INFORMATION

Standard Name NUP82
Systematic Name YJL061W
Alias HRB187
Feature Type ORF, Verified
Description Nucleoporin, subunit of the nuclear pore complex (NPC); forms a subcomplex with Gle2p, Nup159p, Nsp1p, and Nup116p and is required for proper localization of Nup116p in the NPC (1, 2 and see Summary Paragraph)
Name Description NUclear Pore
GO Annotations All NUP82 GO evidence and references
    View Computational GO annotations for NUP82
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Mutant Phenotype All NUP82 Phenotype details and references
Classical genetics
reduction of function
Large-scale survey
null
Interactions NUP82 All interactions details and references
79 total interaction(s) for 30 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 32
  • Affinity Capture-Western: 8
  • Co-localization: 1
  • Co-purification: 2
  • FRET: 5
  • PCA: 7
  • Reconstituted Complex: 2
  • Two-hybrid: 9
Genetic Interactions
  • Dosage Rescue: 3
  • Phenotypic Enhancement: 1
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 8
Sequence Information
ChrX:320010 to 322151 | ORF Map | GBrowse
Gbrowse
Last Update Coordinates: 2009-02-18 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..2142 320010..322151 2009-02-18 1996-07-31
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003597

NUP82 RESOURCES

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SGD ORF map
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  • Localization Resources
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Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for NUP82

SUMMARY PARAGRAPH for NUP82

NUP82 encodes an essential nuclear pore protein that was first isolated by its physical association with Nsp1p (3, 4, 5). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 5, 6, 7, 8). The structure of the vertebrate NPC has been studied extensively; recent reviews include 9, 10, 11, and 12. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (13, 14). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 5, 6, 15, 16, 17). The Nup82p-Nsp1p subcomplex also includes Nup159p (18), and is found exclusively at the cytoplasmic periphery of the nuclear pore (19). This complex is distinct from the subcomplex comprising Nsp1p, Nup49p, Nup57p, and Nic96p (5). PI,PF,PH,PR, At the Nup82Delta108 restrictive temperature, Nup159p is lost from the nuclear pore (18). Mutations in NUP82 are synthetically lethal with mutations in several other nucleoporin genes (5).

Last updated: 1999-07-30

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for NUP82]

1) Ho AK, et al.  (2000) Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p. Mol Cell Biol 20(15):5736-48
2) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
3) Grandi P, et al.  (1995) A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J Cell Biol 130(6):1263-73
4) Hurwitz ME and Blobel G  (1995) NUP82 is an essential yeast nucleoporin required for poly(A)+ RNA export. J Cell Biol 130(6):1275-81
5) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
6) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
7) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
8) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
9) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
10) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
11) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
12) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
13) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
14) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
15) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
16) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
17) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
18) Belgareh N, et al.  (1998) Functional characterization of a Nup159p-containing nuclear pore subcomplex. Mol Biol Cell 9(12):3475-92
19) Fahrenkrog B, et al.  (1998) Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol 143(3):577-88