NUP82/YJL061W Summary Help

Standard Name NUP82 1
Systematic Name YJL061W
Alias HRB187
Feature Type ORF, Verified
Description Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p); relocalizes to the cytosol in response to hypoxia (2, 3, 4, 5, 6, 7, 8, 9 and see Summary Paragraph)
Name Description NUclear Pore 1
Chromosomal Location
ChrX:320016 to 322157 | ORF Map | GBrowse
Gene Ontology Annotations All NUP82 GO evidence and references
  View Computational GO annotations for NUP82
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 1 genes
Classical genetics
reduction of function
Large-scale survey
reduction of function
127 total interaction(s) for 55 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 63
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 9
  • Co-localization: 1
  • Co-purification: 2
  • Far Western: 1
  • FRET: 5
  • PCA: 7
  • Reconstituted Complex: 5
  • Two-hybrid: 17

Genetic Interactions
  • Dosage Rescue: 3
  • Negative Genetic: 2
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 10

Expression Summary
Length (a.a.) 713
Molecular Weight (Da) 82,085
Isoelectric Point (pI) 5.09
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrX:320016 to 322157 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..2142 320016..322157 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003597

NUP82 encodes an essential nuclear pore protein that was first isolated by its physical association with Nsp1p (1, 10, 11). Transport of macromolecules between the nucleus and the cytoplasm of eukaryotic cells occurs through the nuclear pore complex (NPC), a large macromolecular complex that spans the nuclear envelope (reviewed in 11, 12, 13, 14). The structure of the vertebrate NPC has been studied extensively; recent reviews include 15, 16, 17, and 18. The yeast NPC shares several features with the vertebrate NPC, despite being smaller and less elaborate (19, 20). Many yeast nuclear pore proteins, or nucleoporins, have been identified by a variety of genetic approaches (reviewed in 11, 12, 21, 22, 23). The Nup82p-Nsp1p subcomplex also includes Nup159p (24), and is found exclusively at the cytoplasmic periphery of the nuclear pore (25). This complex is distinct from the subcomplex comprising Nsp1p, Nup49p, Nup57p, and Nic96p (11). PI,PF,PH,PR, At the Nup82Delta108 restrictive temperature, Nup159p is lost from the nuclear pore (24). Mutations in NUP82 are synthetically lethal with mutations in several other nucleoporin genes (11).

Last updated: 1999-07-30 Contact SGD

References cited on this page View Complete Literature Guide for NUP82
1) Grandi P, et al.  (1995) A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J Cell Biol 130(6):1263-73
2) Ho AK, et al.  (2000) Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p. Mol Cell Biol 20(15):5736-48
3) Rout MP, et al.  (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
4) Hurwitz ME, et al.  (1998) Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex. Proc Natl Acad Sci U S A 95(19):11241-5
5) Belanger KD, et al.  (2004) The karyopherin Msn5/Kap142 requires Nup82 for nuclear export and performs a function distinct from translocation in RPA protein import. J Biol Chem 279(42):43530-9
6) Lutzmann M, et al.  (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
7) West M, et al.  (2007) Novel interaction of the 60S ribosomal subunit export adapter Nmd3 at the nuclear pore complex. J Biol Chem 282(19):14028-37
8) Alber F, et al.  (2007) The molecular architecture of the nuclear pore complex. Nature 450(7170):695-701
9) Ghosh Dastidar R, et al.  (2012) The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2(1):30
10) Hurwitz ME and Blobel G  (1995) NUP82 is an essential yeast nucleoporin required for poly(A)+ RNA export. J Cell Biol 130(6):1275-81
11) Fabre E and Hurt E  (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet 31:277-313
12) Wente SR, et al.  (1997) "The nucleus and nucleocytoplasmic transport in Saccharomyces cerevisiae." Pp. 471-546 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology, edited by Pringle JR, Broach JR and Jones EW. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
13) Pemberton LF, et al.  (1998) Transport routes through the nuclear pore complex. Curr Opin Cell Biol 10(3):392-9
14) Izaurralde E and Adam S  (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA 4(4):351-64
15) Hinshaw JE  (1994) Architecture of the nuclear pore complex and its involvement in nucleocytoplasmic transport. Biochem Pharmacol 47(1):15-20
16) Pante N and Aebi U  (1996) Molecular dissection of the nuclear pore complex. Crit Rev Biochem Mol Biol 31(2):153-99
17) Davis LI  (1995) The nuclear pore complex. Annu Rev Biochem 64:865-96
18) Pante N and Aebi U  (1994) Toward the molecular details of the nuclear pore complex. J Struct Biol 113(3):179-89
19) Rout MP and Blobel G  (1993) Isolation of the yeast nuclear pore complex. J Cell Biol 123(4):771-83
20) Yang Q, et al.  (1998) Three-dimensional architecture of the isolated yeast nuclear pore complex: functional and evolutionary implications. Mol Cell 1(2):223-34
21) Doye V and Hurt E  (1997) From nucleoporins to nuclear pore complexes. Curr Opin Cell Biol 9(3):401-11
22) Doye V and Hurt EC  (1995) Genetic approaches to nuclear pore structure and function. Trends Genet 11(6):235-41
23) Newmeyer DD  (1993) The nuclear pore complex and nucleocytoplasmic transport. Curr Opin Cell Biol 5(3):395-407
24) Belgareh N, et al.  (1998) Functional characterization of a Nup159p-containing nuclear pore subcomplex. Mol Biol Cell 9(12):3475-92
25) Fahrenkrog B, et al.  (1998) Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J Cell Biol 143(3):577-88