ZAP1/YJL056C Summary

ZAP1 BASIC INFORMATION

Standard Name	ZAP1 1
Systematic Name	YJL056C
Alias	ZRG10 2
Feature Type	ORF, Verified
Description	Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains (1, 3, 4, 5)
Name Description	Zinc-responsive Activator Protein 1

GO Annotations	All ZAP1 GO evidence and references
	View Computational GO annotations for ZAP1
Molecular Function
Manually curated	DNA binding (IDA) specific RNA polymerase II transcription factor activity (IMP, ISS)
Biological Process
Manually curated	cellular zinc ion homeostasis (IMP) regulation of transcription from RNA polymerase II promoter (IMP)
Cellular Component
Manually curated	nucleus (IDA)

Regulatory Role
Binding motifs	ZAP1 Transcription Factor Binding Sites and References
	ACCCTMAAGGTYRT ACCYYNAAGGT

Mutant Phenotype	All ZAP1 Phenotype details and references
Classical genetics
null	Slt2p modification: increased resistance to caffeine: decreased
Large-scale survey
null	acid pH resistance: decreased alkaline pH resistance: decreased glycogen accumulation: increased competitive fitness: decreased resistance to caffeine: decreased resistance to cycloheximide: decreased resistance to hydroxyurea: decreased resistance to hygromycin B: decreased resistance to oleate: decreased resistance to Loperamide: decreased resistance to ethylenediaminetetraacetic acid: decreased resistance to 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to isopropyl (N-3-chloro-phenyl)-carbamate: decreased resistance to diltiazem-HCl: decreased resistance to polymyxin B: decreased resistance to 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid: decreased resistance to lovastatin: decreased utilization of carbon source: decreased utilization of iron source: decreased vegetative growth: decreased viable
overexpression	vegetative growth: decreased

Interactions	ZAP1 All interactions details and references
	10 total interaction(s) for 9 unique genes/features.
Physical Interactions	Affinity Capture-MS: 1 Affinity Capture-RNA: 1 Protein-RNA: 1 Two-hybrid: 2
Genetic Interactions	Phenotypic Enhancement: 1 Phenotypic Suppression: 1 Synthetic Lethality: 3

Sequence Information

ChrX:333067 to 330425 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2009-02-18 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..2643	333067..330425	2009-02-18	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003592

ZAP1 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for ZAP1

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for ZAP1]

1)	Zhao H and Eide DJ (1997) Zap1p, a metalloregulatory protein involved in zinc-responsive transcriptional regulation in Saccharomyces cerevisiae. Mol Cell Biol 17(9):5044-52
2)	Yuan DS (2000) Zinc-regulated genes in Saccharomyces cerevisiae revealed by transposon tagging. Genetics 156(1):45-58
3)	Zhao H, et al. (1998) Regulation of zinc homeostasis in yeast by binding of the ZAP1 transcriptional activator to zinc-responsive promoter elements. J Biol Chem 273(44):28713-20
4)	Evans-Galea MV, et al. (2003) Two of the five zinc fingers in the Zap1 transcription factor DNA binding domain dominate site-specific DNA binding. Biochemistry 42(4):1053-61
5)	Wu CY, et al. (2009) Repression of sulfate assimilation is an adaptive response of yeast to the oxidative stress of zinc deficiency. J Biol Chem 284(40):27544-56

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