VPS53/YJL029C Summary Help

Standard Name VPS53 1
Systematic Name YJL029C
Feature Type ORF, Verified
Description Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p, (1, 2, 3, 4)
Name Description Vacuolar Protein Sorting
Chromosomal Location
ChrX:390853 to 388385 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Gene Ontology Annotations All VPS53 GO evidence and references
  View Computational GO annotations for VPS53
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Regulators 3 genes
Resources
Classical genetics
null
Large-scale survey
null
overexpression
Resources
132 total interaction(s) for 81 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 17
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 15
  • Biochemical Activity: 1
  • Co-fractionation: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 12

Genetic Interactions
  • Negative Genetic: 4
  • Phenotypic Enhancement: 21
  • Phenotypic Suppression: 32
  • Synthetic Growth Defect: 16
  • Synthetic Lethality: 8
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 822
Molecular Weight (Da) 95,448
Isoelectric Point (pI) 5.32
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrX:390853 to 388385 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..2469 390853..388385 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003566
References cited on this page View Complete Literature Guide for VPS53
1) Conibear E and Stevens TH  (2000) Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell 11(1):305-23
2) Siniossoglou S and Pelham HR  (2001) An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes. EMBO J 20(21):5991-8
3) Siniossoglou S and Pelham HR  (2002) Vps51p links the VFT complex to the SNARE Tlg1p. J Biol Chem 277(50):48318-24
4) Dotiwala F, et al.  (2013) DNA damage checkpoint triggers autophagy to regulate the initiation of anaphase. Proc Natl Acad Sci U S A 110(1):E41-9