Standard Name	VPS53 1
Systematic Name	YJL029C
Feature Type	ORF, Verified
Description	Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting (1, 2, 3)
Name Description	Vacuolar Protein Sorting

Chromosomal Location	ChrX:390853 to 388385 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.

	View Computational GO annotations for VPS53
Molecular Function
Manually curated	molecular_function unknown (ND)
Biological Process
Manually curated	Golgi to vacuole transport (IMP) retrograde transport, endosome to Golgi (IDA)
Cellular Component
Manually curated	GARP complex (IPI) Golgi apparatus (IDA, TAS)
High-throughput	cytoplasm (IDA)

Classical genetics
null	lithium(1+) accumulation: increased ionic stress resistance: decreased killer toxin resistance: decreased meiosis: abnormal carboxypeptidase Y secretion: decreased Ape1p (API) modification: normal Ape1p (API) modification: absent resistance to gentamycin: decreased resistance to calcium dichloride: decreased sporulation efficiency: abnormal toxin resistance: increased vacuolar morphology: abnormal viability: decreased
Large-scale survey
null	cell size: increased glycogen accumulation: decreased rubidium(1+) accumulation: increased glutathione excretion: increased competitive fitness: decreased haploinsufficient heat sensitivity: increased ionic stress resistance: decreased metal resistance: decreased oxidative stress resistance: decreased resistance to streptomycin: decreased resistance to cycloheximide: decreased resistance to fenpropimorph: increased resistance to fluconazole: increased resistance to sirolimus: decreased resistance to arsenite(3-): decreased resistance to quinine: decreased resistance to 4-chlorophenol: decreased resistance to bleomycin: decreased resistance to caffeine: decreased resistance to hydroxyurea: decreased resistance to tunicamycin: decreased resistance to dimethyl sulfoxide: decreased resistance to chloroquine: decreased resistance to mefloquine: decreased resistance to wortmannin: normal resistance to cycloheximide: increased resistance to calcium dichloride: decreased resistance to hygromycin B: decreased resistance to sorbate: decreased respiratory growth: decreased rate respiratory growth: absent sporulation: abnormal sporulation: decreased stress resistance: decreased toxin resistance: increased viable
overexpression	filamentous growth: decreased
Resources	PROPHECY | SCMD | ScreenTroll | Yeast Fitness Database

	130 total interaction(s) for 79 unique genes/features.
Physical Interactions	Affinity Capture-MS: 17 Affinity Capture-RNA: 2 Affinity Capture-Western: 15 Biochemical Activity: 1 Co-fractionation: 1 Reconstituted Complex: 1 Two-hybrid: 12
Genetic Interactions	Negative Genetic: 4 Phenotypic Enhancement: 21 Phenotypic Suppression: 32 Synthetic Growth Defect: 14 Synthetic Lethality: 8 Synthetic Rescue: 2
Resources	BioGRID | BOND | BioPIXIE | CYC2008 (complexes) | Complexome | DIP | DRYGIN | GeneMANIA | InterologFinder

Resources	Expression Summary | Cell cycle and metabolic oscillation | Cell cycle transcript profile | Cyclebase | Genevestigator | GermOnline | SPELL | YMGV | YeastFunc

Localization	YeastRC | Organelle DB (UMich) | YPL+ | YeastGFP
Phosphorylation	PhosphoGRID | PhosphoPep Database
Structure	GPMDB | SCOP | YeastRC
Homologs	Ashbya (AGD) | CGD Homologs | CandidaDB Homologs | Fungal Orthogroups Repository | P-POD | PDB Homologs | PhylomeDB | YGOB | YOGY

	ChrX:390853 to 388385 | ORF Map | GBrowse
	Note: this feature is encoded on the Crick strand.
Last Update	Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details	Relative Coordinates Chromosomal Coordinates Most Recent Updates Coordinates Sequence CDS 1..2469 390853..388385 2011-02-03 1996-07-31
Retrieve sequences	S.c. Reference Strain S288C S.c. Strain AWRI1631 S.c. Strain AWRI796 S.c. Strain BY4741 S.c. Strain BY4742 S.c. Strain CBS7960 S.c. Strain CEN.PK113-7D S.c. Strain CLIB215 S.c. Strain CLIB324 S.c. Strain EC1118 S.c. Strain EC9-8 S.c. Strain FostersB S.c. Strain FostersO S.c. Strain JAY291 S.c. Strain Kyokai7 S.c. Strain LalvinQA23 S.c. Strain PW5 S.c. Strain RM11-1a S.c. Strain Sigma1278b S.c. Strain T7 S.c. Strain UC5 S.c. Strain Vin13 S.c. Strain VL3 S.c. Strain W303 S.c. Strain Y10 S.c. Strain YJM269 S.c. Strain YJM789 S.c. Strain YPS163 S.c. Strain ZTW1 ORF Genomic DNA ORF Translation Coding sequence (CDS)

S288C only	BLASTP | ATCC/WashU Clones | BLASTN | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species	Fungal Alignment | BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Synteny Viewer
S288C vs. other strains	Strain Alignment

External Links	All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB

Primary SGDID	S000003566