BUD32/YGR262C Summary

BUD32/YGR262C Summary

Standard Name	BUD32 1
Systematic Name	YGR262C
Alias	LDB14
Feature Type	ORF, Verified
Description	Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription (1, 2, 3, 4, 5)
Name Description	BUD site selection 1

Chromosomal Location	ChrVII:1017759 to 1016974 \| ORF Map \| GBrowse
	Note: this feature is encoded on the Crick strand.

Gene Ontology Annotations All BUD32 GO evidence and references

	View Computational GO annotations for BUD32
Molecular Function
Manually curated	protein serine/threonine kinase activity (IDA)
Biological Process
Manually curated	positive regulation of transcription from RNA polymerase II promoter (IPI) protein phosphorylation (IDA) telomere maintenance (IGI, IMP) threonylcarbamoyladenosine metabolic process (IMP)
High-throughput	cellular bud site selection (IMP)
Cellular Component
Manually curated	EKC/KEOPS complex (IDA)
High-throughput	cytoplasm (IDA) nucleus (IDA)

Mutant phenotypes All BUD32 Phenotype evidence and references

Classical genetics
null	PTR2 mRNA accumulation: decreased PMA1 mRNA accumulation: increased tRNA-Ile modification: absent acid pH resistance: decreased hyperosmotic stress resistance: decreased ionic stress resistance: decreased alpha-factor secretion: decreased precursor carboxypeptidase Y accumulation: increased Ptr2p-GFP distribution: abnormal resistance to canavanine: increased resistance to monensin: decreased resistance to caffeine: decreased resistance to sirolimus: decreased small molecule transport: decreased vegetative growth: decreased
reduction of function	tRNA-Ile modification: decreased vegetative growth: decreased
Large-scale survey
null	acid pH resistance: decreased bud morphology: abnormal budding pattern: abnormal cell size: increased proton accumulation: decreased competitive fitness: decreased heat sensitivity: increased lipid particle morphology: abnormal nutrient utilization: decreased resistance to L-1,4-dithiothreitol: decreased resistance to sirolimus: decreased resistance to sorbate: decreased resistance to quinine: decreased resistance to pyrrolidine dithiocarbamate: decreased resistance to hydrogen chloride: decreased resistance to acetic acid: decreased resistance to (S)-lactic acid: decreased resistance to glucose: decreased resistance to wortmannin: decreased resistance to hydroxyurea: decreased resistance to hygromycin B: decreased respiratory growth: absent respiratory growth: decreased rate sporulation: absent starvation resistance: decreased toxin resistance: increased vegetative growth: decreased rate viable
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All BUD32 Interaction evidence and references

	136 total interaction(s) for 99 unique genes/features.
Physical Interactions	Affinity Capture-MS: 39 Affinity Capture-RNA: 3 Affinity Capture-Western: 7 Biochemical Activity: 5 Far Western: 2 PCA: 1 Reconstituted Complex: 3 Two-hybrid: 11
Genetic Interactions	Dosage Growth Defect: 34 Dosage Lethality: 1 Negative Genetic: 22 Phenotypic Suppression: 1 Synthetic Growth Defect: 2 Synthetic Haploinsufficiency: 1 Synthetic Lethality: 1 Synthetic Rescue: 3
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All BUD32 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP \| YeastRC
Homologs	Ashbya (AGD) \| CGD Homologs \| CandidaDB Homologs \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:1017759 to 1016974 | |

Note: this feature is encoded on the Crick strand.

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..786	1017759..1016974	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003494

References cited on this page View Complete Literature Guide for BUD32

1)	Ni L and Snyder M (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2)	Stocchetto S, et al. (1997) Biochemical evidence that Saccharomyces cerevisiae YGR262c gene, required for normal growth, encodes a novel Ser/Thr-specific protein kinase. FEBS Lett 414(1):171-5
3)	Downey M, et al. (2006) A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator. Cell 124(6):1155-68
4)	Kisseleva-Romanova E, et al. (2006) Yeast homolog of a cancer-testis antigen defines a new transcription complex. EMBO J 25(15):3576-85
5)	Srinivasan M, et al. (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30(5):873-81