PUP2/YGR253C Summary

PUP2 BASIC INFORMATION

Standard Name	PUP2
Systematic Name	YGR253C
Alias	DOA5
Feature Type	ORF, Verified
Description	Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta (1, 2, 3)
Name Description	PUtative Proteasome subunit

GO Annotations	All PUP2 GO evidence and references
	View Computational GO annotations for PUP2
Molecular Function
Manually curated	endopeptidase activity (TAS)
Biological Process
Manually curated	ascospore formation (TAS) response to stress (TAS) ubiquitin-dependent protein catabolic process (TAS)
Cellular Component
Manually curated	proteasome core complex, alpha-subunit complex (IPI, TAS) proteasome storage granule (IDA)

Mutant Phenotype	All PUP2 Phenotype details and references
Large-scale survey
conditional	heat sensitivity: increased
null	inviable

Interactions	PUP2 All interactions details and references
	112 total interaction(s) for 44 unique genes/features.
Physical Interactions	Affinity Capture-MS: 38 Affinity Capture-RNA: 1 Affinity Capture-Western: 8 Biochemical Activity: 1 Co-crystal Structure: 2 Co-purification: 1 PCA: 1 Reconstituted Complex: 3 Two-hybrid: 2
Genetic Interactions	Synthetic Growth Defect: 23 Synthetic Rescue: 32

Sequence Information

ChrVII:999145 to 998363 | |

Note: this feature is encoded on the Crick strand.

Last Update

Coordinates: 2005-11-29 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..783	999145..998363	2005-11-29	1996-07-31

External Links	All Associated Seq \| E.C. \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003485

PUP2 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PUP2

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PUP2]

1)	Groll M, et al. (1997) Structure of 20S proteasome from yeast at 2.4 A resolution. Nature 386(6624):463-71
2)	Georgatsou E, et al. (1992) Molecular cloning of an essential yeast gene encoding a proteasomal subunit. FEBS Lett 299(1):39-43
3)	Velichutina I, et al. (2004) Plasticity in eucaryotic 20S proteasome ring assembly revealed by a subunit deletion in yeast. EMBO J 23(3):500-10

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