NAS6/YGR232W Summary Help

Standard Name NAS6 1
Systematic Name YGR232W
Feature Type ORF, Verified
Description Assembly chaperone for the 19S proteasome regulatory particle base; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6 (1, 2, 3, 4, 5)
Name Description Non-ATPase Subunit 6 1
Chromosomal Location
ChrVII:953960 to 954646 | ORF Map | GBrowse
Gbrowse
Gene Ontology Annotations All NAS6 GO evidence and references
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 8 genes
Resources
Classical genetics
null
Large-scale survey
null
Resources
157 total interaction(s) for 45 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 84
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 35
  • Co-crystal Structure: 2
  • Co-purification: 1
  • PCA: 1
  • Reconstituted Complex: 1
  • Two-hybrid: 5

Genetic Interactions
  • Dosage Lethality: 1
  • Negative Genetic: 4
  • Phenotypic Enhancement: 5
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 1
  • Synthetic Rescue: 2

Resources
Expression Summary
histogram
Resources
Length (a.a.) 228
Molecular Weight (Da) 25,616
Isoelectric Point (pI) 6.42
Localization
Phosphorylation PhosphoGRID | PhosphoPep Database
Structure
Homologs
sequence information
ChrVII:953960 to 954646 | ORF Map | GBrowse
SGD ORF map
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates
Chromosomal
Coordinates
Most Recent Updates
Coordinates Sequence
CDS 1..687 953960..954646 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
Resources
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003464
References cited on this page View Complete Literature Guide for NAS6
1) Hori T, et al.  (1998) cDNA cloning and functional analysis of p28 (Nas6p) and p40.5 (Nas7p), two novel regulatory subunits of the 26S proteasome. Gene 216(1):113-22
2) Padmanabhan B, et al.  (2004) Crystal structure of the homolog of the oncoprotein gankyrin, an interactor of Rb and CDK4/6. J Biol Chem 279(2):1546-52
3) Roelofs J, et al.  (2009) Chaperone-mediated pathway of proteasome regulatory particle assembly. Nature 459(7248):861-5
4) Saeki Y, et al.  (2009) Multiple proteasome-interacting proteins assist the assembly of the yeast 19S regulatory particle. Cell 137(5):900-13
5) Funakoshi M, et al.  (2009) Multiple assembly chaperones govern biogenesis of the proteasome regulatory particle base. Cell 137(5):887-99