PET54/YGR222W Summary

PET54 BASIC INFORMATION

Standard Name	PET54 1
Systematic Name	YGR222W
Feature Type	ORF, Verified
Description	Mitochondrial inner membrane protein that binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing (1, 2, 3, 4)
Name Description	PETite colonies

GO Annotations	All PET54 GO evidence and references
	View Computational GO annotations for PET54
Molecular Function
Manually curated	RNA binding (IDA) translation regulator activity (IMP)
Biological Process
Manually curated	Group I intron splicing (IDA, IGI) positive regulation of mitochondrial translation (IMP)
Cellular Component
Manually curated	mitochondrial inner membrane (IDA) mitochondrial matrix (IDA)
High-throughput	mitochondrion (IDA)

Mutant Phenotype	All PET54 Phenotype details and references
Classical genetics
null	petite Cox3p accumulation: absent respiratory growth: absent
Large-scale survey
null	glycogen accumulation: decreased competitive fitness: decreased resistance to 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine: decreased respiratory growth: decreased rate respiratory growth: absent viable
overexpression	vegetative growth: decreased

Interactions	PET54 All interactions details and references
	11 total interaction(s) for 7 unique genes/features.
Physical Interactions	Protein-RNA: 2 Two-hybrid: 4
Genetic Interactions	Dosage Rescue: 2 Synthetic Growth Defect: 1 Synthetic Rescue: 2

Sequence Information

ChrVII:939926 to 940807 | |

Genetic position: 174 cM

Last Update

Coordinates: 2004-07-20 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..882	939926..940807	2004-07-20	1996-07-31

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| UniProtKB

Primary SGDID	S000003454

PET54 RESOURCES

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SGD ORF map	GBrowse

Click on histogram for expression summary
Expression Summary

ADDITIONAL INFORMATION for PET54

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for PET54]

1)	Costanzo MC, et al. (1989) The PET54 gene of Saccharomyces cerevisiae: characterization of a nuclear gene encoding a mitochondrial translational activator and subcellular localization of its product. Genetics 122(2):297-305
2)	Valencik ML, et al. (1989) Disruption of the yeast nuclear PET54 gene blocks excision of mitochondrial intron aI5 beta from pre-mRNA for cytochrome c oxidase subunit I. EMBO J 8(12):3899-904
3)	Naithani S, et al. (2003) Interactions among COX1, COX2, and COX3 mRNA-specific translational activator proteins on the inner surface of the mitochondrial inner membrane of Saccharomyces cerevisiae. Mol Biol Cell 14(1):324-33
4)	Kaspar BJ, et al. (2008) A shared RNA-binding site in the Pet54 protein is required for translational activation and group I intron splicing in yeast mitochondria. Nucleic Acids Res 36(9):2958-68

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