CCH1/YGR217W Summary Help

Standard Name CCH1
Systematic Name YGR217W
Feature Type ORF, Verified
Description Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together (1, 2, 3, 4, 5, 6 and see Summary Paragraph)
Chromosomal Location
ChrVII:924696 to 930815 | ORF Map | GBrowse
Gene Ontology Annotations All CCH1 GO evidence and references
  View Computational GO annotations for CCH1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 4 genes
Classical genetics
reduction of function
Large-scale survey
63 total interaction(s) for 51 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 5
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 1

Genetic Interactions
  • Dosage Lethality: 1
  • Dosage Rescue: 1
  • Negative Genetic: 39
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 1
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 5
  • Synthetic Rescue: 1

Expression Summary
Length (a.a.) 2,039
Molecular Weight (Da) 234,597
Isoelectric Point (pI) 8.5
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:924696 to 930815 | ORF Map | GBrowse
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..6120 924696..930815 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003449

CCH1 encodes a high-affinity calcium channel with homology to mammalian voltage-gated calcium channels (7). Based on mutant evidence and coimmunoprecipiation data, Cch1p is thought to function together with the stretch-activated cation channel Mid1p to form a channel for Ca2+ influx at the plasma membrane (2). Along with the transporters Pmr1p, Pmc1p and the vacuolar ion exchanger Vcx1p, Cch1p and Mid1p help regulate cellular calcium levels and mediate calcium signaling.

Ca2+ influx is stimulated by pheromone treatment (see 8 and references therein), hypertonic stress (4), ER stress including accumulation of misfolded proteins in the ER (3), the antiarrhythmic and fungicidal drug amiodarone (9), alkaline stress (5), and iron stress (6). Null mutations in CCH1 or MID1 cause hypersensitivity to these conditions, and result in an inability to recover from long-term treatment with alpha factor pheromone (1, 10).

CCH1 homologs have been described in Cryptococcus (11) and in plants (12, 13) as well as in mammals (14). Mutations in the human homolog CACNA1A are associated with spinocerebellar ataxia 6 (15), familial hemiplegic migraine (16), and episodic ataxia-2 (16).

Last updated: 2006-12-05 Contact SGD

References cited on this page View Complete Literature Guide for CCH1
1) Fischer M, et al.  (1997) The Saccharomyces cerevisiae CCH1 gene is involved in calcium influx and mating. FEBS Lett 419(2-3):259-62
2) Locke EG, et al.  (2000) A homolog of voltage-gated Ca(2+) channels stimulated by depletion of secretory Ca(2+) in yeast. Mol Cell Biol 20(18):6686-94
3) Bonilla M, et al.  (2002) Essential role of calcineurin in response to endoplasmic reticulum stress. EMBO J 21(10):2343-53
4) Matsumoto TK, et al.  (2002) An osmotically induced cytosolic Ca2+ transient activates calcineurin signaling to mediate ion homeostasis and salt tolerance of Saccharomyces cerevisiae. J Biol Chem 277(36):33075-80
5) Viladevall L, et al.  (2004) Characterization of the calcium-mediated response to alkaline stress in Saccharomyces cerevisiae. J Biol Chem 279(42):43614-24
6) Peiter E, et al.  (2005) The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity. FEBS Lett 579(25):5697-703
7) Paidhungat M and Garrett S  (1997) A homolog of mammalian, voltage-gated calcium channels mediates yeast pheromone-stimulated Ca2+ uptake and exacerbates the cdc1(Ts) growth defect. Mol Cell Biol 17(11):6339-47
8) Muller EM, et al.  (2001) Differential regulation of two Ca(2+) influx systems by pheromone signaling in Saccharomyces cerevisiae. Genetics 159(4):1527-38
9) Gupta SS, et al.  (2003) Antifungal activity of amiodarone is mediated by disruption of calcium homeostasis. J Biol Chem 278(31):28831-9
10) Iida H, et al.  (1994) MID1, a novel Saccharomyces cerevisiae gene encoding a plasma membrane protein, is required for Ca2+ influx and mating. Mol Cell Biol 14(12):8259-71
11) Liu M, et al.  (2006) Cch1 mediates calcium entry in Cryptococcus neoformans and is essential in low-calcium environments. Eukaryot Cell 5(10):1788-96
12) Kadota Y, et al.  (2004) Identification of putative voltage-dependent Ca2+-permeable channels involved in cryptogein-induced Ca2+ transients and defense responses in tobacco BY-2 cells. Biochem Biophys Res Commun 317(3):823-30
13) Hashimoto K, et al.  (2004) Functional analysis of a rice putative voltage-dependent Ca2+ channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1. Plant Cell Physiol 45(4):496-500
14) Diriong S, et al.  (1995) Chromosomal localization of the human genes for alpha 1A, alpha 1B, and alpha 1E voltage-dependent Ca2+ channel subunits. Genomics 30(3):605-9
15) Zhuchenko O, et al.  (1997) Autosomal dominant cerebellar ataxia (SCA6) associated with small polyglutamine expansions in the alpha 1A-voltage-dependent calcium channel. Nat Genet 15(1):62-9
16) Ophoff RA, et al.  (1996) Familial hemiplegic migraine and episodic ataxia type-2 are caused by mutations in the Ca2+ channel gene CACNL1A4. Cell 87(3):543-52