MVB12/YGR206W Summary

MVB12/YGR206W Summary

Standard Name	MVB12 1, 2
Systematic Name	YGR206W
Feature Type	ORF, Verified
Description	ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin (1, 2, 3, 4, 5)
Name Description	MultiVesicular Body sorting factor of 12 kilodaltons 1

Chromosomal Location	ChrVII:910432 to 910737 \| ORF Map \| GBrowse

Gene Ontology Annotations All MVB12 GO evidence and references

	View Computational GO annotations for MVB12
Molecular Function
Manually curated	ubiquitin binding (IDA)
Biological Process
Manually curated	endosome transport via multivesicular body sorting pathway (IC) negative regulation of protein complex assembly (IMP) protein targeting to vacuole (IMP)
Cellular Component
Manually curated	cytosol (IDA) endosome (IDA) ESCRT I complex (IDA, IPI)
High-throughput	endosome (IDA)

Mutant phenotypes All MVB12 Phenotype evidence and references

Classical genetics
overexpression	nuclear morphology: abnormal
Large-scale survey
null	competitive fitness: decreased endocytosis: decreased resistance to (S)-lactic acid: increased resistance to nickel sulfate: increased resistance to acetic acid: increased resistance to sirolimus: decreased resistance to ethanol: decreased resistance to wortmannin: normal resistance to methyl methanesulfonate: decreased resistance to dimethyl sulfoxide: decreased stress resistance: increased toxin resistance: increased vacuolar morphology: abnormal viable
overexpression	cell cycle progression: abnormal
Resources	PROPHECY \| SCMD \| ScreenTroll \| Yeast Fitness Database

interactions All MVB12 Interaction evidence and references

	35 total interaction(s) for 30 unique genes/features.
Physical Interactions	Affinity Capture-MS: 5 Affinity Capture-RNA: 1 Affinity Capture-Western: 2
Genetic Interactions	Negative Genetic: 20 Phenotypic Enhancement: 2 Positive Genetic: 2 Synthetic Growth Defect: 2 Synthetic Lethality: 1
Resources	BioGRID \| BOND \| BioPIXIE \| CYC2008 (complexes) \| Complexome \| DIP \| DRYGIN \| GeneMANIA \| InterologFinder

Expression Summary


Resources	Expression Summary \| Cell cycle and metabolic oscillation \| Cell cycle transcript profile \| Cyclebase \| Genevestigator \| GermOnline \| SPELL \| YMGV \| YeastFunc

Protein Information All MVB12 Protein evidence and references

Localization	YeastRC \| YPL+ \| YeastGFP
Phosphorylation	PhosphoGRID \| PhosphoPep Database
Structure	GPMDB \| SCOP
Homologs	Ashbya (AGD) \| Fungal Orthogroups Repository \| P-POD \| PDB Homologs \| PhylomeDB \| YGOB \| YOGY

sequence information

ChrVII:910432 to 910737 | |

SGD ORF map

Last Update

Coordinates: 2011-02-03 | Sequence: 1996-07-31

Subfeature details

	Relative Coordinates	Chromosomal Coordinates	Most Recent Updates
	Relative Coordinates	Chromosomal Coordinates	Coordinates	Sequence
CDS	1..306	910432..910737	2011-02-03	1996-07-31

Retrieve sequences

Analyze Sequence

S288C only	BLASTP \| ATCC/WashU Clones \| BLASTN \| Design Primers \| Restriction Fragment Map \| Restriction Fragment Sizes \| Six-Frame Translation
S288C vs. other species	Fungal Alignment \| BLASTN vs. fungi \| BLASTP at NCBI \| BLASTP vs. fungi \| Synteny Viewer
S288C vs. other strains	Strain Alignment

Resources

External Links	All Associated Seq \| Entrez Gene \| Entrez RefSeq Protein \| MIPS \| Search all NCBI (Entrez) \| UniProtKB

Primary SGDID	S000003438

References cited on this page View Complete Literature Guide for MVB12

1)	Curtiss M, et al. (2007) Efficient cargo sorting by ESCRT-I and the subsequent release of ESCRT-I from multivesicular bodies requires the subunit Mvb12. Mol Biol Cell 18(2):636-45
2)	Chu T, et al. (2006) New component of ESCRT-I regulates endosomal sorting complex assembly. J Cell Biol 175(5):815-23
3)	Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91
4)	Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91
5)	Xie MW, et al. (2005) Insights into TOR function and rapamycin response: chemical genomic profiling by using a high-density cell array method. Proc Natl Acad Sci U S A 102(20):7215-20