PCT1/YGR202C Summary Help

Standard Name PCT1 1
Systematic Name YGR202C
Alias BSR2 , CCT1 2
Feature Type ORF, Verified
Description Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity (2, 3, 4, 5, 6, 7)
Name Description Phosphocholine CytidylylTransferase 8
Chromosomal Location
ChrVII:904748 to 903474 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gene Ontology Annotations All PCT1 GO evidence and references
  View Computational GO annotations for PCT1
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
Regulators 2 genes
Classical genetics
Large-scale survey
87 total interaction(s) for 51 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 7
  • Affinity Capture-RNA: 1
  • PCA: 1
  • Two-hybrid: 1

Genetic Interactions
  • Dosage Rescue: 1
  • Negative Genetic: 48
  • Phenotypic Enhancement: 5
  • Phenotypic Suppression: 6
  • Positive Genetic: 6
  • Synthetic Growth Defect: 6
  • Synthetic Rescue: 5

Expression Summary
Length (a.a.) 424
Molecular Weight (Da) 49,406
Isoelectric Point (pI) 9.26
Phosphorylation PhosphoGRID | PhosphoPep Database
sequence information
ChrVII:904748 to 903474 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Last Update Coordinates: 2011-02-03 | Sequence: 1996-07-31
Subfeature details
Most Recent Updates
Coordinates Sequence
CDS 1..1275 904748..903474 2011-02-03 1996-07-31
Retrieve sequences
Analyze Sequence
S288C only
S288C vs. other species
S288C vs. other strains
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | Search all NCBI (Entrez) | UniProtKB
Primary SGDIDS000003434
References cited on this page View Complete Literature Guide for PCT1
1) Xie Z, et al.  (1998) Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects. Proc Natl Acad Sci U S A 95(21):12346-51
2) Tsukagoshi Y, et al.  (1987) Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae. Eur J Biochem 169(3):477-86
3) Skinner HB, et al.  (1995) The Saccharomyces cerevisiae phosphatidylinositol-transfer protein effects a ligand-dependent inhibition of choline-phosphate cytidylyltransferase activity. Proc Natl Acad Sci U S A 92(1):112-6
4) Sreenivas A, et al.  (1998) A role for phospholipase D (Pld1p) in growth, secretion, and regulation of membrane lipid synthesis in yeast. J Biol Chem 273(27):16635-8
5) Dowd SR, et al.  (2001) Turnover of phosphatidylcholine in Saccharomyces cerevisiae. The role of the CDP-choline pathway. J Biol Chem 276(6):3756-63
6) Friesen JA, et al.  (2001) Purification and kinetic characterization of CTP:phosphocholine cytidylyltransferase from Saccharomyces cerevisiae. Protein Expr Purif 21(1):141-8
7) Ding Z, et al.  (2012) A 22-mer segment in the structurally pliable regulatory domain of metazoan CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions. J Biol Chem 287(46):38980-91
8) Johnson JE, et al.  (1992) Comparison of the lipid regulation of yeast and rat CTP: phosphocholine cytidylyltransferase expressed in COS cells. Biochem J 285 ( Pt 3)():815-20