TDH3/YGR192C Summary Help

TDH3 BASIC INFORMATION

Standard Name TDH3
Systematic Name YGR192C
Alias GLD1 , HSP35 , HSP36 , SSS2
Feature Type ORF, Verified
Description Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall (1, 2 and see Summary Paragraph)
Name Description Triose-phosphate DeHydrogenase 2
Gene Product Alias GPD
GO Annotations All TDH3 GO evidence and references
    View Computational GO annotations for TDH3
Molecular Function
Manually curated
Biological Process
Manually curated
Cellular Component
Manually curated
High-throughput
Pathways
Mutant Phenotype All TDH3 Phenotype details and references
Classical genetics
null
Large-scale survey
null
Interactions TDH3 All interactions details and references
58 total interaction(s) for 46 unique genes/features.
Physical Interactions
  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 1
  • Co-fractionation: 1
  • Co-purification: 1
  • Two-hybrid: 4
Genetic Interactions
  • Dosage Rescue: 2
  • Phenotypic Suppression: 3
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 4
Sequence Information
ChrVII:883815 to 882817 | ORF Map | GBrowse
Note: this feature is encoded on the Crick strand.
Gbrowse
Last Update Coordinates: 2004-07-20 | Sequence: 1996-07-31
Subfeature details
Relative
Coordinates		 Chromosomal
Coordinates		 Most Recent Updates
Coordinates Sequence
CDS 1..999 883815..882817 2004-07-20 1996-07-31
External Links All Associated Seq | E.C. | Entrez Gene | Entrez RefSeq Protein | MIPS | UniProtKB
Primary SGDIDS000003424

TDH3 RESOURCES

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Click on histogram for expression summary
Expression Summary histogram

ADDITIONAL INFORMATION for TDH3

SUMMARY PARAGRAPH for TDH3

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer catalyzing the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate. Three unlinked genes, TDH1, TDH2, and TDH3, encode related but not identical polypeptides that form catalytically active homotetramers with different specific activities (2), 1). Interestingly, these catalytically active enzymes are found in the cytoplasm and cell wall. Tdh2p and Tdh3p are detected in exponentially growing cells whereas Tdh1p is primarily detected during stationary phase (3). Therefore, it has been suggested, but not confirmed, that Tdh1p may be involved in a process other than glycolysis because it is synthesized by cells in stationary phase (4).

Glyceraldehyde-3-phosphate dehydrogenase activity is also required during gluconeogenesis, which is the process whereby glucose is synthesized from non-carbohydrate precursors, enabling yeast cells to grow on ethanol, glycerol, or peptone.

The reactions of gluconeogenesis, shown here, mediate conversion of pyruvate to glucose, which is the opposite of glycolysis, the formation of pyruvate from glucose. While these two pathways have several reactions in common, they are not the exact reverse of each other. As the glycolytic enzymes phosphofructokinase (Pfk1p, Pfk2p) and pyruvate kinase (Cdc19p) only function in the forward direction, the gluconeogenesis pathway replaces those steps with the enzymes pyruvate carboxylase (Pyc1p, Pyc2p) and phosphoenolpyruvate carboxykinase (Pck1p) -generating oxaloacetate as an intermediate from pyruvate to phosphoenolpyruvate- and also the enzyme fructose-1,6-bisphosphatase (Fbp1p) (reviewed in 5). Overall, the gluconeogenic reactions convert two molecules of pyruvate to a molecule of glucose, with the expenditure of six high-energy phosphate bonds, four from ATP and two from GTP.

The TDH3 promoter is often referred to as the GPD promoter, which is used in the p4XX series of expression vectors (6).

Last updated: 2008-12-12

REFERENCES CITED ON THIS PAGE [View Complete Literature Guide for TDH3]

1) McAlister L and Holland MJ  (1985) Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260(28):15019-27
2) McAlister L and Holland MJ  (1985) Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260(28):15013-8
3) Delgado ML, et al.  (2001) The glyceraldehyde-3-phosphate dehydrogenase polypeptides encoded by the Saccharomyces cerevisiae TDH1, TDH2 and TDH3 genes are also cell wall proteins. Microbiology 147(Pt 2):411-7
4) Boucherie H, et al.  (1995) Differential synthesis of glyceraldehyde-3-phosphate dehydrogenase polypeptides in stressed yeast cells. FEMS Microbiol Lett 125(2-3):127-33
5) Klein CJ, et al.  (1998) Glucose control in Saccharomyces cerevisiae: the role of Mig1 in metabolic functions. Microbiology 144 ( Pt 1):13-24
6) Mumberg D, et al.  (1995) Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 156(1):119-22